| 人GDNF基因在昆虫细胞中的高效表达 |
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| 引用本文： | 陈哲宇,孙建新.人GDNF基因在昆虫细胞中的高效表达[J].生物化学与生物物理学报,1999,31(3):245-253. |
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| 作者姓名： | 陈哲宇 孙建新 |
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| 作者单位： | [1]上海第二军医大学神经生物学教研室 [2]中国科学院上海生物化学研究所 |
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| 摘 要： | 应用昆虫杆状病毒表达系统在昆虫细胞Ｔｎ－５Ｂ１－４中高效表达了人胶质细胞源性神经营养因子（ＧＤＮＦ），ＰＡＧＥ分析表达量占细胞可溶性蛋白质的３０％左右，表达产物经亲和层析纯化后纯度达８０％以上，活性研究表明，昆虫细胞表达的ＧＤＮＦ蛋白能显著促进多巴胺能神经元的存活，此研究为进一步研究ＧＤＮＦ结构与功能打下了良好的基础。
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| 关 键 词： | 重组 胶质细胞 神经营养因子 昆虫杆状病毒 表达 |
Cloning of Human Glial Neurotrophic Factor and Its High Expression in Insect Cells |
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| CHEN Zhe,Yu and LU Chang,Lin.Cloning of Human Glial Neurotrophic Factor and Its High Expression in Insect Cells[J].Acta Biochimica et Biophysica Sinica,1999,31(3):245-253. |
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| Authors: | CHEN Zhe Yu and LU Chang Lin |
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| Abstract: | The human glial cell derived neurotrophic factor(GDNF)was expressed in Tn 5B1 4 insect cells using the Bac to Bac baculovirus expression system, accounting for 30% of total cellular soluble proteins. The expressed product was purified by affinity chromatography and the activity assays showed that it could significantly promote the survival of dopaminergic neurons. The study can be the basis for further structural functional analysis of GDNF. |
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| Keywords: | Recombinant GDNF baculovirus expression system(BES) dopaminergic neuron |
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