| 一种新的烙铁头蛇毒肌肉毒素 |
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| 引用本文： | 徐天瑞,王婉瑜.一种新的烙铁头蛇毒肌肉毒素[J].生物化学与生物物理学报,1999,31(5):483-488. |
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| 作者姓名： | 徐天瑞 王婉瑜 |
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| 作者单位： | 中国科学院昆明动物研究所!昆明650223 |
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| 摘 要： | 用分子筛和快速蛋白质液相色谱从烙铁头（ＴＲｒｉｍｅｒｅｓｕｒｕｓ ｍｕｃｒｏｓｑｕａｍａｔｕｓ）蛇毒中分离了一个新的碱性肌肉毒素，命名为ＴＭＰＢ。它的分子量为１６０００，等电点为９．２．用蛋白质序列仪测定了其Ｎ端２４个氨基酸残基，ＴＭＰＢ与其他两个从同种蛇毒中分离到的碱性磷酯酶Ａ２的同源性分别为４１．７％和５４．２％《
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| 关 键 词： | 肌肉毒素 磷酯酶A2 烙铁头蛇 蛇毒 |
A Novel Myotoxin from the Venom of Trimeresurus mucrosquamatus |
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| XU Tian Rui,WANG Wan Yu,MENG Qing Xiong,HUANG Yu Hui,LU Qiu Ming and XIONG Yu Liang.A Novel Myotoxin from the Venom of Trimeresurus mucrosquamatus[J].Acta Biochimica et Biophysica Sinica,1999,31(5):483-488. |
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| Authors: | XU Tian Rui WANG Wan Yu MENG Qing Xiong HUANG Yu Hui LU Qiu Ming and XIONG Yu Liang |
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| Institution: | XU Tian Rui,WANG Wan Yu,MENG Qing Xiong,HUANG Yu Hui,LU Qiu Ming and XIONG Yu Liang * |
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| Abstract: | A novel myotoxin, designated TMPB, was purified from the venom of Trimeresurus mucrosquamatus by Sephadex G 100 superfine gel chromatography and fast protein liquid chromatography (FPLC). The N terminal sequence of 24 amino acid residues was determined by protein sequencer. The sequence similarities between TMPB and other two phospholipase A 2 (PLA 2s) previously purified from the same venom were 41.7% and 54.2%, respectively, but TMPB showed no detectable PLA 2 hydrolytic activity. Its molecular weight was estimated to be 16 000 by reducing SDS PAGE and isoelectric point was determined to be 9.2 by isoelectric focusing electrophoresis. TMPB exhibited strong myotoxicity and platelet aggregation inhibiting activity, and the two activities could all be inhibited by heparin. |
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| Keywords: | Myotoxin phospholipase A 2 platelet Trimeresurus mucrosquamatus venom |
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