新城疫病毒融合蛋白基因部分序列分析

Ｆ蛋白是ＮＤＶ中具有抗原性的两种表面糖蛋白之一，通过ＲＴ－ＰＣＲ，克隆了速发现ＮＤＶ中国株Ｆ４８Ｅ８的Ｆ基因，比较分析Ｆ４８Ｅ８，ＡＵＳ和ＩＴＡ等速发型株系Ｆ基因的Ｎ端结构，氨基酸同源性分别达９５％和９３．６％，变化主要集中于信号肽区，成熟蛋白质的同源性极高。该基因的克隆为研制ＮＤＶ的基因工程疫苗打下基础。

Analysis of N Terminal Nucleotide Sequence of NDV Fusion Protein

Fusion protein of Newcastle disease virus is a glycoprotein with antigenicity. The cDNA of fusion gene of NDV strain F48E8 was cloned by RT PCR, and the sequence of F48E8 was compared with other two virulent strains, Australia victoria and Italien. Results show that the sequences of N terminus are very similar, and cysteine residues and the potential glycosylation sites are relatively conserved. However, there are a few differences in the region of signal peptides.