剪切应力下内皮细胞内皮素及其mRNA的表达

应用Ｎｏｒｔｈｅｒｎ印迹方法研究高血压（ＳＨＲ）和正常（ＷＫＹ）大鼠脑微血管培养内皮细胞，在剪切应力０、０．５、１、２Ｐａ作用下２４ｈ后检测内皮素及其基因ｍＲＮＡ表达上的区别。结果表明，在剪切应力０、０．５、１Ｐａ下，ＷＫＹ大鼠的内皮细胞随着剪切应力加大，其内皮素水平及其基因的ｍＲＮＡ的表达均比ＷＫＹ相应组的为高。在剪切应力２Ｐａ时，ＷＫＹ和ＳＨＲ大鼠的内皮细胞内皮素及其基因的ｍＲＮＡ表达水平不同

Influence of shearing Stress to the Expression of Endothelin by Vascular Endothelial Cells

Using radioimmunoassay and Northern blot, the differences in endothelin and its mRNA expression were studied for cultured cerebral micro vascular endothelial cells (from WKY and SHR rats) which exposed to flow shear stress at 0, 0.5, 1, 2 Pa for 24 h. The results showed that the endothelin and its mRNA expression of endothelial cells from both WKY and SHR rats proportionally increased according to the shear stress values at 0, 0.5 and 1 Pa, at 2 Pa, however the endothelin and its mRNA expression did not increase further. When comparing both rat systems, the SHR's levels of endothelin and its mRNA expression were higher than WKY's. The results indicated that the levels of endothelin and its mRNA expression were proportional to shear stress in 0-1 Pa in WKY and SHR rat systems and the levels of endothelin and its mRNA of SHR system were higher than those of WKY's. Our results indicate that the vascular wall in SHR system may be more contractile, less relaxative, with lower remodeling ability and thus more easily to mediate atherosclerosis.