| 逆转录病毒载体介导胸苷磷酸化酶在胰腺癌细胞表达 |
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| 引用本文： | 张雷,刘彤华.逆转录病毒载体介导胸苷磷酸化酶在胰腺癌细胞表达[J].生物化学与生物物理学报,1999,31(3):320-324. |
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| 作者姓名： | 张雷 刘彤华 |
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| 作者单位： | 中国科学院上海生物化学研究所，中国农业科学院蚕业研究所 |
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| 摘 要： | 人胸腺嘧啶核苷磷酸化酶（ＴＰ）在一些肿瘤组织中活性增高，但其功能目前了解尚少。构建了表达ＴＰ的重组逆转录病毒载体，直接导入人胰腺癌ＰＣ－２细胞，ｍＰＣＲ扩增、Ｓｏｕｔｈｅｒｎ及Ｎｏｒｔｈｅｒｎ印迹和原位杂交证实转染细胞有外源ＴＰ的整合及表达，酶活性检测发现含外源ＴＰ细胞ＴＰ活性比ＰＣ－２细胞的内源性ＴＰ活性高ＴＰ活性高４０－７０倍，生长曲线和＾３Ｈ－ＴｄＲ参入率检测未发现含外源ＴＰ细胞生物学行为的
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| 关 键 词： | 胸腺嘧啶 核苷磷酸化酶 逆转录病毒 胰腺癌 |
Transgenic Silkworm of Anti NPV Ribozyme |
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| ZHANG Feng,CHEN Xiu ,ZHAO Yun,QI
Guo Rong,HUANG Jun Ting and LU Chang De.Transgenic Silkworm of Anti NPV Ribozyme[J].Acta Biochimica et Biophysica Sinica,1999,31(3):320-324. |
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| Authors: | ZHANG Feng CHEN Xiu ZHAO Yun QI
Guo Rong HUANG Jun Ting and LU Chang De |
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| Institution: | ZHANG Feng,CHEN Xiu 1,ZHAO Yun,QI
Guo Rong,HUANG Jun Ting 1 and LU Chang De |
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| Abstract: | The plasmid pGL2Rz including ribozyme gene was linearized and introduced into early eggs of silkworm (G 0) by gene gun. The luciferase activity in blood of the G 1 generation was detected, then the resistant silkworm was selected by NPV infection from G 2 generation. The transgenic silkworm resistant against NPV 10 times more than control ones was got at the G 4 generation. PCR and Southern blotting proved that the ribozyme gene was integrated into the genome of silkworm multicopily. The expression of ribozyme was also detected by RT PCR in pupa. The results showed that the transgenic silkworm strain of anti NPV ribozyme has been got. |
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| Keywords: | Silkworm ribozyme NPV transgene |
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