Comparison of transformation efficiency of piggyBac transposon among three different silkworm Bombyx mori Strains |
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Authors: | Zhong Boxiong Li Jianying Chen Jin'e Ye Jian Yu Songdong |
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Institution: | College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. bxzhong@zju.edu.cn |
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Abstract: | The transformation rate of three different strains of silkworm Bombyx mori was compared after the introduction of enhanced green fluorescence protein (EGFP)-encoding genes into the silkworm eggs by microinjection of a mixture of piggyBac vector and helper plasmid containing a transposase-encoding sequence.Although there were no significant differences among the three strains in the percentages of fertile moths in microinjected eggs (P=0.1258),the percentages of G_0 transformed moths in fertile moths and injected eggs were both significantly different (P=0.01368 and P=0.02398, respectively).The transformation rate of the Nistari strain (Indian strain) was significantly higher than that of the other two strains,Golden-yellow-cocoon (Vietnamese strain) and Jiaqiu (Chinese strain),which had similar rate. These results indicate that the transformation efficiency of the piggyBac-based system might vary with silkworm strains with different genetic backgrounds.The presence of endogenous piggyBac-like elements might be an important factor influencing the transformation efficiency of introduced piggyBac-derived vectors,and the diverse amount and activation in different silkworm strains might account for the significant differences. |
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Keywords: | Bombyx mori piggyBac transposon transgenesis transformation efficiency |
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