一个新的受雄激素诱导的胞浆蛋白的组织分布

本文以３２Ｐ－５＇末端标记ＰＳＢＰ－Ｃ３基因启动子的３１ｂｐＤＮＡ片段作探针，用凝胶阻滞分析法检测了大鼠、小鼠、兔和猴各组织以及人病理组织的胞浆中与此ＤＮＡ片段特异结合的新蛋白质（Ｃ３Ｐ４），以期对其功能的探讨提供必要的线索。结果显示：（１）Ｃ３Ｐ４蛋白以不同的含量存在于４种动物的雄性器官中；（２）４种动物的脑组织均含有此蛋白质，其他组织中很难检测到，且其结果因种族而异：（３）雌性动物中此蛋白质的组织分布除性器官外，与雄性动物相同；（４）在所测定的８种人器官的几种病理组织中，初步观察到胃癌中含量较高，乳房的良性和恶性肿瘤中都有Ｃ２Ｐ４蛋白存在，７例前列腺肥大中有３例比较明显。

The Tissue Distribution of a New Androgen Inducible Cytosol Protein

As a preliminary but important step in the investigation of the function of the new androgen-dependent DNA binding cytosol protein (C3P4) discovered by using gel retardation assay with a 31 bp fragment of DNA of the promoter of the rat ventral prostatic binding protein (PSBP) O3 gene as a probe, a general survey was carried out on the tissue distribution of this protein in rat, mice, rabbit and monkey of both sexes and also in some human pathological tissues with the following findings: (1) C3P4 is present in all the male organs in different amount, but none in female sex organs. (2) O3P4 has been found in the brain of all the species studied. (3) Its occurrence in other tissues are rare and show species variation, but no sex difference. (4) of the samples collected from 8 different human pathological tissues, stomach cancer, both benign and malignant mammary tumors and three of the benign prostotic hyperplasia tissues revealed certain changes, the potential significance of these findings were discussed.