| DNA Vaccine of SARS-Cov S Gene Induces Antibody Response in Mice |
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| 作者姓名： | Zhao P Ke JS Qin ZL Ren H Zhao LJ Yu JG Gao J Zhu SY Qi ZT |
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| 作者单位： | Department of Microbiology，Second Military Medical University，Shanghai 200433，China; Department of Infectious Diseases，PLA Hospital No.88，Tai'an 271000，China |
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| 基金项目： | This work was supported by grants from National Key Basic Research and Development Program (No.2003CB514129)，SMMU Special Funds for SARS Prophylaxis and Treatment (No.2003SARS03) |
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| 摘 要： | The spike (S) protein, a main surface antigen of SARS-coronavirus (SARS-CoV), is one of themost important antigen candidates for vaccine design. In the present study, three fragments of the truncated S protein were expressed in E. coli, and analyzed with pooled sera of convalescence phase of SARS patients. The full length S gene DNA vaccine was constructed and used to immunize BALB/c mice. The mouse serum IgG antibody against SARS-CoV was measured by ELISA with E. coli expressed truncated S protein or SARS-CoV lysate as diagnostic antigen. The results showed that all the three fragments of S protein expressed by E. coli was able to react with sera of SARS patients and the S gene DNA candidate vaccine could induce the production of specific IgG antibody against SARS-CoV efficiently in mice with seroconversion ratio of 75% after 3 times of immunization. These findings lay some foundations for further understanding the immunology of SARS-CoV and developing SARS vaccines.
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| 关 键 词： | 小鼠 免疫反应 抗体 基因疫苗 SARS 严重急性呼吸综合征 冠状病毒 |
DNA vaccine of SARS-Cov S gene induces antibody response in mice |
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| Zhao P,Ke JS,Qin ZL,Ren H,Zhao LJ,Yu JG,Gao J,Zhu SY,Qi ZT.DNA Vaccine of SARS-Cov S Gene Induces Antibody Response in Mice[J].Acta Biochimica et Biophysica Sinica,2004,36(1):37-41. |
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| Authors: | Zhao Ping Ke Jin-Shan Qin Zhao-Lin Ren Hao Zhao Lan-Juan Yu Jian-Guo Gao Jun Zhu Shi-Ying Qi Zhong-Tian |
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| Institution: | Department of Microbiology, Second Military Medical University, Shanghai 200433, China. |
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| Abstract: | The spike (S) protein, a main surface antigen of SARS-coronavirus (SARS-CoV), is one of the most important antigen candidates for vaccine design. In the present study, three fragments of the truncated S protein were expressed in E. coli, and analyzed with pooled sera of convalescence phase of SARS patients. The full length S gene DNA vaccine was constructed and used to immunize BALB/c mice. The mouse serum IgG antibody against SARS-CoV was measured by ELISA with E. coli expressed truncated S protein or SARS-CoV lysate as diagnostic antigen. The results showed that all the three fragments of S protein expressed by E. coli was able to react with sera of SARS patients and the S gene DNA candidate vaccine could induce the production of specific IgG antibody against SARS-CoV efficiently in mice with seroconversion ratio of 75% after 3 times of immunization. These findings lay some foundations for further understanding the immunology of SARS-CoV and developing SARS vaccines. |
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| Keywords: | severe acute respiratory syndrome-coronavirus (SARS-CoV) spike protein DNA vaccine antibody |
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