| 严重急性呼吸综合征（SARS）病毒的形态及其形态发生机制 |
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| 引用本文： | 张勤奋,崔金明,黄小俊,林伟,谭冬艳,徐洁薇,杨艺峰,张景强,张欣,李晖,郑焕英,陈秋霞,鄢心革,郑夔,万卓越,黄吉城.严重急性呼吸综合征（SARS）病毒的形态及其形态发生机制[J].生物化学与生物物理学报,2003,35(6):587-591. |
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| 作者姓名： | 张勤奋 崔金明 黄小俊 林伟 谭冬艳 徐洁薇 杨艺峰 张景强 张欣 李晖 郑焕英 陈秋霞 鄢心革 郑夔 万卓越 黄吉城 |
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| 作者单位： | 中山大学生物防治国家重点实验室 广州510275
(张勤奋，崔金明，黄小俊，林伟，谭冬艳，徐洁薇，杨艺峰，张景强)，广东省疾病预防与控制中心 广州510300
(张欣，李晖，郑焕英，陈秋霞，鄢心革，郑夔，万卓越)，广东省疾病预防与控制中心 广州510300(黄吉城) |
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| 基金项目： | 广东省非典型性肺炎科技攻关资助课题~~ |
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| 摘 要： | 将SARS患者的咽拭子感染VeroE6细胞 ,用电子显微技术等对SARS病毒进行了研究。结果表明 ,新分离到的病毒粒子没带囊膜时直径大多约 5 0nm ,带有囊膜的直径约 10 0nm。通过RT PCR等证明 ,该病毒是新的冠状病毒。这些病毒可与SARS康复患者的血清呈强烈的阳性反应 ,表明此新的冠状病毒是引起SARS的主要病原。文中还对病毒的发生机制和细胞中的分布进行了探讨。
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| 关 键 词： | 严重急性呼吸综合征 冠状病毒 电子显微技术 |
Morphology and Morphogenesis of Severe Acute Respiratory Syndrome (SARS)-associated Virus |
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| ZHANG Qin-Fen,CUI Jin-Ming,HUANG Xiao-Jun,LIN Wei,TAN Dong-Yan,XU Jie-Wei,YANG Yi-Feng,ZHANG Jing-Qiang.Morphology and Morphogenesis of Severe Acute Respiratory Syndrome (SARS)-associated Virus[J].Acta Biochimica et Biophysica Sinica,2003,35(6):587-591. |
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| Authors: | ZHANG Qin-Fen CUI Jin-Ming HUANG Xiao-Jun LIN Wei TAN Dong-Yan XU Jie-Wei YANG Yi-Feng ZHANG Jing-Qiang |
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| Institution: | ZHANG Qin-Fen,CUI Jin-Ming,HUANG Xiao-Jun,LIN Wei,TAN Dong-Yan,XU Jie-Wei,YANG Yi-Feng,ZHANG Jing-Qiang * |
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| Abstract: | After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article. |
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| Keywords: | severe acute respiratory syndrome (SARS) coronavirus electron microscopy |
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