| 17个新的C2H2型锌指基因片段的分离与克隆 |
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| 引用本文： | 张民,余龙.17个新的C2H2型锌指基因片段的分离与克隆[J].生物化学与生物物理学报,1998,30(5):465-470. |
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| 作者姓名： | 张民 余龙 |
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| 作者单位： | 复旦大学遗传学研究所遗传工程国家重点实验室 |
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| 基金项目： | 国家863高技术项目,国家杰出青年科学基金,国家自然科学基金 |
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| 摘 要： | 按照Ｃ２Ｈ２型锌指基因保守结构域的ＤＮＡ序列设计一对简并引物，以人基因组ＤＮＡ为模板进行ＰＣＲ同源扩增，将由此获得的锌指基因片段为探针，从人胎肾、骨骼肌、骨骼组织的ｃＤＮＡ分子库中筛选到２２个Ｃ２Ｈ２型锌指蛋白ｃＤＮＡ片段，经国际ＮＣＢＩ数据库查询检索，其中１７个为新的锌指基因片段。对从胎肾ｃＤＮＡ分子库中分离到的Ｋ３－４和Ｋ５－１２克隆进行了表达谱分析，发现Ｋ３－４在肾脏中的表达量明显高于其他几
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| 关 键 词： | C2H2型锌指基因 同源扩增 表达谱分析 克隆 |
Isolation and Cloning of 17 Novel C 2H 2 Zinc Finger Gene Fragments |
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| ZHANG Min,YU Long ,HU Pei Rong,WANG Xiao Ke,BI An Ding,CHEN Qi and ZHAO Shou Yuan.Isolation and Cloning of 17 Novel C 2H 2 Zinc Finger Gene Fragments[J].Acta Biochimica et Biophysica Sinica,1998,30(5):465-470. |
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| Authors: | ZHANG Min YU Long HU Pei Rong WANG Xiao Ke BI An Ding CHEN Qi and ZHAO Shou Yuan |
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| Institution: | ZHANG Min,YU Long *,HU Pei Rong,WANG Xiao Ke,BI An Ding,CHEN Qi and ZHAO Shou Yuan |
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| Abstract: | A pair of degenerate primers were designed according to the DNA sequences of the C 2H 2 zinc finger genes conservative domain and then homologious PCR was performed with the human genomic total DNA as template. The zinc finger fragments thus obtained were used as probes to screen cDNA libraries of human fetal kidney, muscle and marrow and 22 C 2H 2 zinc finger gene fragments were selected. Among these fragments, 17 were confirmed to be novel zinc finger gene fragments by literature searches in the National Center Biotechnology Information (NCBI) database. Expression patterns of the clones K3 4 and K5 12 selected from kidney cDNA library were analyzed . The results showed that the expression level of K3 4 in kidney is obviously higher than in other tissues and K5 12 is expressed at different levels in 8 tissues. |
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| Keywords: | C 2H 2 zinc finger gene homogenetic amplification expression pattern analysis |
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