| 天花粉蛋白R122G突变体的构建及活性研究 |
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| 引用本文： | 袁惠东,柯一保.天花粉蛋白R122G突变体的构建及活性研究[J].生物化学与生物物理学报,1998,30(3):311-313. |
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| 作者姓名： | 袁惠东 柯一保 |
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| 作者单位： | 中国科学院上海细胞生物学研究所，中国科学院上海生物化学研究所 |
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| 摘 要： | 天花粉蛋白Ｒ１２２Ｇ突变体的构建及活性研究袁惠东１柯一保孔梅柯欣永夏其昌１张祖传１聂慧玲＊（中国科学院上海细胞生物学研究所，上海２０００３１；１中国科学院上海生物化学研究所，上海２０００３１）关键词天花粉蛋白；突变体；ＲＮＡＮ－糖苷酶天花粉蛋白（ｔｒ...
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| 关 键 词： | 天药粉蛋白 突变体 RNA 糖苷酶 构建 活性 |
Construction of Trichosanthin Mutant R122G and Its Activity Study |
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| YUAN Hui Dong ,KE Yi Bao,KONG Mei,KE Xin Yong ,XIA Qi Chang ,ZHANG Zu Chuan and NIE Hui Ling.Construction of Trichosanthin Mutant R122G and Its Activity Study[J].Acta Biochimica et Biophysica Sinica,1998,30(3):311-313. |
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| Authors: | YUAN Hui Dong KE Yi Bao KONG Mei KE Xin Yong XIA Qi Chang ZHANG Zu Chuan and NIE Hui Ling |
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| Institution: | YUAN Hui Dong 1,KE Yi Bao,KONG Mei,KE Xin Yong 1,XIA Qi Chang 1,ZHANG Zu Chuan and NIE Hui Ling * |
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| Abstract: | AGG, the codon of 122 Arg of trichosanthin(TCS) was mutated to GGG(Gly) by U DNA site directed mutagenesis. The mutant TCS was expressed and purified from the supernatant of host cells , its activities were assayed and compared with expressed wild type TCS. The results showed that the R122G TCS was still active as a RNA N glycosidase but its ability to inhibit protein synthesis was 160 fold less than that of wild type TCS , its abortifacient ability on mid term gestated mice reduced from 100% to 9.3%, which suggested that 122Arg was indirectly involved in the catalysis, and it played an important role in its bioactivities. |
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| Keywords: | Trichosanthin mutant RNA N glycosidase |
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