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天花粉蛋白R122G突变体的构建及活性研究
引用本文:袁惠东 柯一保. 天花粉蛋白R122G突变体的构建及活性研究[J]. Acta biochimica et biophysica Sinica, 1998, 30(3): 311-313
作者姓名:袁惠东 柯一保
作者单位:中国科学院上海细胞生物学研究所,中国科学院上海生物化学研究所
摘    要:天花粉蛋白R122G突变体的构建及活性研究袁惠东1柯一保孔梅柯欣永夏其昌1张祖传1聂慧玲*(中国科学院上海细胞生物学研究所,上海200031;1中国科学院上海生物化学研究所,上海200031)关键词天花粉蛋白;突变体;RNAN-糖苷酶天花粉蛋白(tr...

关 键 词:天药粉蛋白 突变体 RNA 糖苷酶 构建 活性

Construction of Trichosanthin Mutant R122G and Its Activity Study
YUAN Hui Dong ,KE Yi Bao,KONG Mei,KE Xin Yong ,XIA Qi Chang ,ZHANG Zu Chuan and NIE Hui Ling. Construction of Trichosanthin Mutant R122G and Its Activity Study[J]. Acta biochimica et biophysica Sinica, 1998, 30(3): 311-313
Authors:YUAN Hui Dong   KE Yi Bao  KONG Mei  KE Xin Yong   XIA Qi Chang   ZHANG Zu Chuan  NIE Hui Ling
Affiliation:YUAN Hui Dong 1,KE Yi Bao,KONG Mei,KE Xin Yong 1,XIA Qi Chang 1,ZHANG Zu Chuan and NIE Hui Ling *
Abstract:AGG, the codon of 122 Arg of trichosanthin(TCS) was mutated to GGG(Gly) by U DNA site directed mutagenesis. The mutant TCS was expressed and purified from the supernatant of host cells , its activities were assayed and compared with expressed wild type TCS. The results showed that the R122G TCS was still active as a RNA N glycosidase but its ability to inhibit protein synthesis was 160 fold less than that of wild type TCS , its abortifacient ability on mid term gestated mice reduced from 100% to 9.3%, which suggested that 122Arg was indirectly involved in the catalysis, and it played an important role in its bioactivities.
Keywords:Trichosanthin  mutant  RNA N glycosidase  
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