| 硫酸化对金顶侧耳多糖构象及生物活性的影响 |
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| 引用本文： | 张丽萍,张翼伸.硫酸化对金顶侧耳多糖构象及生物活性的影响[J].生物化学与生物物理学报,1994,26(4):417-421. |
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| 作者姓名： | 张丽萍 张翼伸 |
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| 作者单位： | 东北师范大学生物系，白求恩医科大学应用基础研究所病毒室 |
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| 摘 要： | 从金顶侧耳子实体中分离纯化一半乳甘露聚糖ＰＣ－３。该多糖在水中为无规线团构象，与Ｃｏｎ Ａ可相互结合并产生沉淀，对柯萨奇病毒ＣＢ５有一定的抑制作用，ＰＣ－３经硫酸化修饰后，由于同性电荷的排斥作用，使糖链的无规线团扩展呈伸展状态，局部可能形成螺旋。硫酸化的ＰＣ－３与Ｃｏｎ Ａ不能结合成多糖－蛋白复合物，但显著地提高了抗病毒ＣＢ５的活性。
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| 关 键 词： | 金顶侧耳 多糖 硫酸化 构象 食用菌 |
The Influence of Sulfation on the Conformation and Biological Activity of Polysaccharides from Pleurotus citrinopileatus |
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| ZHANG Li-Ping,ZHANG Yi-Shen,SHUN Feiand LIANG Zhong-Yan.The Influence of Sulfation on the Conformation and Biological Activity of Polysaccharides from Pleurotus citrinopileatus[J].Acta Biochimica et Biophysica Sinica,1994,26(4):417-421. |
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| Authors: | ZHANG Li-Ping ZHANG Yi-Shen SHUN Feiand LIANG Zhong-Yan |
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| Abstract: | A D-galacto-D-mannan PC-3,isolated and purified from fruit bodies of Pleurotus citrinopileatus assumes a random coil conformation in aqueous solution.PC-3 can combine with Con A and form precipitations. It exhibits partial antiCoxsackie B5 activity.Sulfation of PC-3 led to a change in its solution conformation,probably due to negatively charge exclusion. The sulfated PC-3 could not combine Con A,and was shown to have increased anti-virus CB5 activity. |
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| Keywords: | Pleurotus citrinopileatus polysaccharide Sulfation Conformation Virus CB_5 |
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