| Oleanolic Acid Induces Apoptosis in Human Leukemia Cells through Caspase Activation and Poly（ADP-ribose） Polymerase Cleavage |
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| 作者姓名： | Zhang P Li H Chen D Ni J Kang Y Wang S |
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| 作者单位： | [1]Department of Histology and Embryology, Jilin University, Jilin 130021, China [2]Pharmacological Research Institute, Qiqihar Medical College, Qiqihar 161024, China [3]Department of Histology and Embryology, Guangdong Medical College, Zhanjiang 524023, China [4]Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China [5]Department of Physiology, Shanxi Medical University, Taiyuan 030001, China [6]School of Basic Science of Medicine, Jiamusi University, Jiamusi 154007, China |
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| 摘 要： | It has been shown that Fructus Ligustri Lucidi （FLL）, a promising traditional Chinese medicine, can inhibit the growth of tumors. However, the effective component and molecular mechanism of FLL act to inhibit tumor proliferation are unclear. In this study, we demonstrated that oleanolic acid （OA）, a principal chemical component of FLL, inhibited the proliferation of human leukemia HL60 cells in culture. MTT assay showed that treatment of HL60 cells with FLL crude extracts or OA dramatically blocked the growth of target tumor cell in a time- and dose-dependent manner. Morphological changes of the nuclei and DNA fragmentation showed that apoptotic cell death occurred in the HL60 cells after treating with FLL extracts （20 mg/ml） or OA （3.65×10^-2 mg/ml）. Furthermore, flow cytometry assay showed that treatment of HL60 cells with FLL or OA caused an increased accumulation of G1 and sub-G1 subpopulations. Western blot analysis showed that caspase-9 and caspase-3 were activated, accompanied by the cleavage of poly （ADP-ribose） polymerase （PARP） in the target cells during FLL- or OA-induced apoptosis, These results suggest that OA acts as the effective component of FLL by exerting its cytotoxicity towards target tumor cells through activation of caspases and cleavage of PARP.
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| 关 键 词： | 白血病 细胞调亡 HL60细胞 聚合酶 |
| 修稿时间： | 2007-03-20 |
Oleanolic acid induces apoptosis in human leukemia cells through caspase activation and poly(ADP-ribose) polymerase cleavage |
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| Zhang P,Li H,Chen D,Ni J,Kang Y,Wang S.Oleanolic acid induces apoptosis in human leukemia cells through caspase activation and poly(ADP-ribose) polymerase cleavage[J].Acta Biochimica et Biophysica Sinica,2007,39(10):803-809. |
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| Authors: | Zhang Pengxia Li Hongmei Chen Dong Ni Juhua Kang Yuming Wang Shuqiu |
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| Institution: | Department of Histology and Embryology, Guangdong Medical College, Zhanjiang 524023, China. |
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| Abstract: | It has been shown that Fructus Ligustri Lucidi (FLL), a promising traditional Chinese medicine, can inhibit the growth of tumors. However, the effective component and molecular mechanism of FLL act to inhibit tumor proliferation are unclear. In this study, we demonstrated that oleanolic acid (OA), a principal chemical component of FLL, inhibited the proliferation of human leukemia HL60 cells in culture. MTT assay showed that treatment of HL60 cells with FLL crude extracts or OA dramatically blocked the growth of target tumor cell in a time- and dose-dependent manner. Morphological changes of the nuclei and DNA fragmentation showed that apoptotic cell death occurred in the HL60 cells after treating with FLL extracts (20 mg/ml) or OA (3.65 x 10(-2) mg/ml). Furthermore, flow cytometry assay showed that treatment of HL60 cells with FLL or OA caused an increased accumulation of G(1) and sub-G(1) subpopulations. Western blot analysis showed that caspase-9 and caspase-3 were activated, accompanied by the cleavage of poly(ADP-ribose) polymerase (PARP) in the target cells during FLL- or OA-induced apoptosis. These results suggest that OA acts as the effective component of FLL by exerting its cytotoxicity towards target tumor cells through activation of caspases and cleavage of PARP. |
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| Keywords: | oleanolic acid apoptosis caspase-3 PARP HL60 cells |
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