| 复乳法制备红细胞代用品 |
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| 引用本文： | 邱伟,孟凡涛,马光辉,苏志国.复乳法制备红细胞代用品[J].生物化学与生物物理学报,2003,35(5):467-472. |
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| 作者姓名： | 邱伟 孟凡涛 马光辉 苏志国 |
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| 作者单位： | 中国科学院过程工程研究所，中国科学院过程工程研究所，中国科学院过程工程研究所，中国科学院过程工程研究所 生化工程国家重点实验室，北京100080，生化工程国家重点实验室，北京100080，生化工程国家重点实验室，北京100080，生化工程国家重点实验室，北京100080 |
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| 基金项目： | 国家自然科学基金资助项目 (No .2 0 13 60 2 0 )~~ |
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| 摘 要： | 以单甲氧基聚乙二醇聚乳酸共聚物 methoxypoly(ethyleneglycol) poly DL lactide,PELA]为膜材 ,用W /O/W的复乳 溶剂扩散法制备了包埋牛血红蛋白 (bovinehemoglobin ,BHb)的微胶囊作为人造红细胞。研究发现复乳过程搅拌分散速率、有机溶剂种类和固化方法对BHb活性有明显影响。当搅拌分散速率小于 90 0 0r/min、以乙酸乙酯为有机相时 ,采用复乳 溶剂扩散法包埋BHb的过程对BHb活性无明显影响。当搅拌分散速率高于 12 0 0 0r/min时 ,BHb活性降低。复乳 溶剂扩散法制备微胶囊过程中固化液体积与微胶囊中BHb活性密切相关 ,通过增大固化液和复乳液的体积比可较好地保持BHb的活性。最后制得了粒径 10 μm左右、包埋率 93%、P50 和Hill系数均接近于天然BHb的微胶囊。
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| 关 键 词： | 人造红细胞 聚乙二醇聚乳酸共聚物 牛血红蛋白 微胶囊 |
The Preparation of an Artificial Red Blood Cell Substitute by W/O/W Double Emulsion Methods |
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| QIU Wei,MENG Fan-Tao,MA Guang-Hui,SU Zhi-Guo.The Preparation of an Artificial Red Blood Cell Substitute by W/O/W Double Emulsion Methods[J].Acta Biochimica et Biophysica Sinica,2003,35(5):467-472. |
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| Authors: | QIU Wei MENG Fan-Tao MA Guang-Hui SU Zhi-Guo |
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| Institution: | QIU Wei,MENG Fan-Tao,MA Guang-Hui,SU Zhi-Guo * |
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| Abstract: | A method of preparing methoxypoly (ethylene glycol)-poly-DL-lactide (PELA) microcapsule containing bovine hemoglobin(BHb) by using W/O/W double emulsion-solvent diffusion process for use as artificial red cells was developed. The results suggested that preparation conditions such as solidification method, stirring rate, type of organic solvent had significant influence on the encapsulation efficiency and the activity of bovine hemoglobin. When ethyl acetate was used as the organic solvent and double emulsion-solvent diffusion process was used, there was no significant influence on the activity of hemoglobin if stirring rate was lower than 9000 r/min. High stirring rate, 12 000 r/min, decreased the P 50 and Hill coefficient of the hemoglobin encapsulated. Increasing the volume of solidification solution had an effect of improving the activity of hemoglobin to carry oxygen. |
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| Keywords: | artificial red cells methoxypoly(ethylene glycol)-poly-DL-lactide bovine hemoglobin microcapsule |
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