| 天花粉蛋白Lys和Arg残基的化学修饰对其与IgE反应活性的影响 |
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| 引用本文： | 何贤辉,柯一保,蔡迎春,聂慧玲.天花粉蛋白Lys和Arg残基的化学修饰对其与IgE反应活性的影响[J].生物化学与生物物理学报,1994(6). |
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| 作者姓名： | 何贤辉 柯一保 蔡迎春 聂慧玲 |
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| 作者单位： | 中国科学院上海细胞生物学研究所 |
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| 摘 要： | 用马来酸酐及环己二酮分别对天花粉蛋白上的Ｌｙｓ、Ａｒｇ残基侧链进行修饰，并以竞争性酶免疫测定检查了化学修饰对ＴＣＳ与小鼠抗ＴＣＳ单抗ＴＥ－１（ＩｇＥ型）反应活性的影响。两种修饰均使ＴＣＳ与单抗ＴＥ－１反应活性下降。推测Ｌｙｓ残基可能直接参予了与单抗ＴＥ－１的结合。热变性实验提示ＴＣＳ上单抗ＴＥ－１识别部位需要一定的空间构象。
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| 关 键 词： | 天花粉蛋白 IgE型单抗 化学修饰 竞争性酶免疫测定 |
Effects of Chemical Modification of Lysine and Arginine Residues of Trichosanthin on its Reactivity with IgE |
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| HE Xian-Hui,KE Yi-Bao,CAI Ying-Chun and NIE Hui-Ling.Effects of Chemical Modification of Lysine and Arginine Residues of Trichosanthin on its Reactivity with IgE[J].Acta Biochimica et Biophysica Sinica,1994(6). |
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| Authors: | HE Xian-Hui KE Yi-Bao CAI Ying-Chun and NIE Hui-Ling |
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| Abstract: | We reported here the modification of lysine and arginine residues of TCS with maleic anhydride and 1,2-cyclohexyldione respectively and their effect on TCS' reactivity with IgE antibody by competitive enzyme immunoassay.The reactivity of TCS with IgE antibody decreased by these two modifications.We presumed that some Lys residues may take part in binding with IgE antibody directly.The result of heat-denaturing experiments showed that there may be certain spatial conformation on TCS molecule necessary for the recognition of the IgE antibody. |
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| Keywords: | Trichosanthin IgE monoclonal antibody Chemical modification Competitive enzyme immunoassay |
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