Surface properties of sendai virus envelope |
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Authors: | Tanveer F Abidi Philip L Yeagle |
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Institution: | Department of Biochemistry, SUNY / Buffalo School of Medicine, Buffalo, NY 14214 U.S.A. |
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Abstract: | Surface properties of Sendai virus envelope membrane have been measured, using both biological and biophysical techniques. Both normal and trypsin-treated virus were studied. SDS gel electrophoresis showed cleavage of the F protein exclusively by trypsin. The major activity change was observed in the hemolysing activity which is an expression of F protein. Hemolysis was reduced to less than 10% of its value for intact virus. 31P nuclear magnetic resonance studies of the envelope surface of the native virus showed a highly restricted phospholipid headgroup environment. Interestingly, this restriction was relieved by treatment with trypsin. Thus these data suggest a role of the F protein of Sendai virus in tightly organizing the surface of the viral envelope membrane. |
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Keywords: | Virus envelope Membrane surface Glycoprotein F Trypsin treatment ESR electron spin resonance NMR nuclear magnetic resonance SDS sodium dodecyl sulfate |
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