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New features of the cell wall of the radio-resistant bacterium Deinococcus radiodurans |
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| Authors: | Domenica Farci Matthew W Bowler Joanna Kirkpatrick Sean McSweeney Enzo Tramontano Dario Piano |
| Institution: | 1. Department of Life and Environmental Sciences, Laboratory of Plant Physiology, University of Cagliari, V.le S. Ingnazio da Laconi 13, 09123 Cagliari, Italy;2. European Molecular Biology Laboratory, Grenoble Outstation, 6 Rue Jules Horowitz, 38042 Grenoble, France;3. Unit for Virus Host Cell Interactions, Univ. Grenoble Alpes-EMBL-CNRS, 6 rue Jules Horowitz, 38042 Grenoble, France;4. European Molecular Biology Laboratory, Meyerhofstraße 1, 69117 Heidelberg, Germany;5. European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, 38000 Grenoble, France;6. Department of Life and Environmental Sciences, Laboratory of Molecular Virology, University of Cagliari, Cittadella Universitaria di Monserrato, SS 554, 09042 Monserrato, Cagliari, Italy |
| Abstract: | We have analyzed the cell wall of the radio-resistant bacterium Deinococcus radiodurans. Unexpectedly, the bacterial envelope appears to be organized in different complexes of high molecular weight. Each complex is composed of several proteins, most of which are coded by genes of unknown function and the majority are constituents of the inner/outer membrane system. One of the most abundant complexes is constituted by the gene DR_0774. This protein is a type of secretin which is a known subunit of the homo-oligomeric channel that represents the main bulk of the type IV piliation family. Finally, a minor component of the pink envelope consists of several inner-membrane proteins. The implications of these findings are discussed. |
| Keywords: | HPI Hexagonally Packed Intermediate SlpA Surface Layer Protein A TGY Tryptone/Glucose/Yeast extract broth β-DDM n-Dodecyl-β-D-maltoside SDS-PAGE Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis BN Blue native Rf Retardation factor DTT Dithiotreitol MS Mass Spectrometry SLH Surface Layer Homology T2SS Type II Secretion System T3SS Type III Secretion System T4P Type IV Piliation System MW Molecular Weight FDR False Discovery Rate DPC DNA Processing Complex |
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