Interactions of glycerol monooleate and dimethylsulphoxide with phospholipids A differential scanning calorimetry and 31P-NMR study |
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Authors: | Colin PS Tilcock Derek Fisher |
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Institution: | Department of Biochemistry and Chemistry, Royal Free Hospital School of Medicine, University of London, 8 Hunter St., London WC1N 1 BP U.K. |
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Abstract: | 1. A comparative study has been made of the effects of the fusogens glycerol monooleate and dimethylsulphoxide on the polymorphic phase behaviour of dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylethanolamine by differential scanning calorimetry and 31P-NMR techniques. 2. Glycerol monooleate induces a reduction in the temperature, cooperativity and enthalpy of the gel to liquid-crystal transitions of dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylethanolamine, whereas dimethylsulphoxide induces an increase in the temperature and enthalpy and a reduction in the cooperativity of the gel to liquid-crystal transitions for those same phospholipids. 3. Glycerol monooleate induces the formation of isotropic and hexagonal (HII) phases when mixed with either dipalmitoyl phosphatidylcholine or dipalmitoyl phosphatidylethanolamine. By contrast, in the presence of dimethylsulphoxide, those same phospholipids retain the lamellar configuration observed in the absence of fusogen. 4. These results are discussed in terms of the mechanisms of chemically induced cell fusion. |
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Keywords: | Glycerol monooleate Dimethylsulfoxide Phospholipid Cell fusion Differential scanning calorimetry DPPC Dipalmitoyl phosphatidylcholine DPPE dipalmitoyl phosphatidylethanolamine DMSO dimethylsulphoxide DSC differential scanning calorimetry |
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