| 从“湖北光敏感核不育水稻”的未受精子房和花药培养出单倍体植株 |
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| 引用本文： | 蔡得田,陈冬玲,祝虹,金聿.从“湖北光敏感核不育水稻”的未受精子房和花药培养出单倍体植株[J].分子细胞生物学报,1988(4). |
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| 作者姓名： | 蔡得田 陈冬玲 祝虹 金聿 |
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| 作者单位： | 华中农业大学农学系 武汉
(蔡得田，陈冬玲，祝虹)，华中农业大学农学系 武汉(金聿) |
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| 摘 要： | 采用10种诱导培养基,培养湖北光敏感核不育水稻农垦58品种的未受精子房和花药。共培养未受精子房2790个,获得胚囊愈伤组织17块,最高诱导频率达3.33%,其中2块分化出绿苗。培养花药16740个,获得花药愈伤组织15块,最高诱导频率为0.92%,其中3块分化出苗,2丛白苗,1株绿苗。胚囊植株和花粉植株经根尖染色体检查为单倍体,2n=x=12。实验证明,液体培养、2,4-D0.2-0.5 mg/1、低温预处理对诱导胚囊愈伤组织及花粉愈伤组织的形成具良好效果。
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In Vitro PRODUCTION OF HAPLOID PLANTLETS FROM THE UNFERTILIZED OVARIES AND ANTHERS OF HUBEI PHOTOSENSITIVE GENIC MALE-STERILE RICE (HPGMR). |
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| Cai De-tian Chen Dong-ling Zhu Hong and Jin Yu.In Vitro PRODUCTION OF HAPLOID PLANTLETS FROM THE UNFERTILIZED OVARIES AND ANTHERS OF HUBEI PHOTOSENSITIVE GENIC MALE-STERILE RICE (HPGMR).[J].Journal of Molecular Cell Biology,1988(4). |
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| Authors: | Cai De-tian Chen Dong-ling Zhu Hong and Jin Yu |
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| Institution: | HuaZhong Agricultural University |
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| Abstract: | |
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| Keywords: | Photosensitive genic male-sterile rice Ovary culture Anther culture Haploid plantlet |
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