| 酵母pro 2基因转化紫云英的研究 |
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| 引用本文： | 李学宝,毛慧珠,白永延.酵母pro 2基因转化紫云英的研究[J].分子细胞生物学报,1997(2). |
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| 作者姓名： | 李学宝 毛慧珠 白永延 |
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| 作者单位： | 华中师范大学生命科学学院，中国科学院植物生理研究所植物分子遗传国家重点实验室，中国科学院植物生理研究所植物分子遗传国家重点实验室 武汉 430070，上海 200032，上海 200032 |
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| 摘 要： | 用外植体一农杆菌共培养法将酵母脯氨酸合成酶基因2(pro 2)导入豆科牧草紫云英,获得转基因植株。Southern blot分析检测到转化植株基因组中存在外源DNA的同源顺序,证明酵母pro 2基因已整合到紫云英细胞基因组中。转化植株具有强的NPT Ⅱ酶活性,而对照植株呈阴性反应。在含0.5%NaCl的培养基上,紫云英植株内游离脯氨酸含量增高,一些转化植株叶片内游离脯氨酸含量显著高于对照,而且耐盐性有所提高。转化植株移栽到土壤中开花结实,收获到R_1代种子。
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| 关 键 词： | 紫云英 酵母pro2基因 转化 耐盐性 |
TRANSFERRING YEAST pro 2 GENE INTO ASTRAGALUS SINICUS TO PRODUCE TRANSGENIC PLANTS USING AGROBACTERIUM TUMEFACIENS |
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| LI Xue Bao MAO Hui Zhu BAI Yong Yan.TRANSFERRING YEAST pro 2 GENE INTO ASTRAGALUS SINICUS TO PRODUCE TRANSGENIC PLANTS USING AGROBACTERIUM TUMEFACIENS[J].Journal of Molecular Cell Biology,1997(2). |
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| Authors: | LI Xue Bao MAO Hui Zhu BAI Yong Yan |
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| Abstract: | The successful transfer of yeast pro 2 gene into Astragalus sinicus via Agrobacte-rium-mediated DNA transfer was demonstrated by DNA Southern blotting. Neomy-cin phosphotransferase gene fused with yeast pro 2 gene a selectable marker, was transferred into Astragalus sinicus and
the expression of the gene in transgenic plants was confirmed by the enzyme activity assay. 7 days after growing under stress at a concentration of 0.5%NaCl, some transgenic plants had higher free pr-oline content and bet ter salt tolerance than control did. |
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| Keywords: | Astragalus sinicus yeast pro 2 gene Transformation Salt tolerance |
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