蚕豆叶肉原生质体电融合的研究

本文研究了蚕豆叶肉原生质体经透明质酸酶、核糖核酸酶、神经氨酸酶、碱性磷酸酶、胰蛋白酶、脂肪酶六种水解酶和SDS、Triton X-100、CTMAB三种表面活性剂以及秋水仙素、细胞松驰素B处理后的电融合过程。结果表明:胰蛋白酶处理后的原生质体融合率明显下降;碱性磷酸酶、脂肪酶以及核糖核酸酶、透明质酸酶、神经氨酸酶处理的原生质体电融合率均有不同程度的上升。Triton X-100和CTMAB促进原生质体的电融合,但较高浓度(0.01%)的SDS起抑制作用。秋水仙素和细胞松驰素B处理的原生质体其电融合率有较大幅度的增高。

STUDIES ON THE ELECTRIC-FUSION OF MESOPHYLL PROTOPLASTS OF VICIA FABA

The electric-fusion of mesophyll proto-plasts of Vicia faba with pretreatment of hya-luronidase,ribonuclease,neuraminidase,alka-line phosphatase,tripsin,lipase and sodiumdodecyl sulfate(SDS),Triton X-100.cetyl-trimethylammonium bromide(CTMAB)andcolchicine,cytochalasin B was studied.Theresults showed that the fusion index wasdecreased with pretreatment of tripsin andincreased with pretreatment of all other hy-drolases.Triton X-100 and CTMAB increasedthe fusion index but higher concentration(0.01%)SDS decreased.Pretreatment of col-chicine and cytochalasin B on the protoplastsmarkedly enhanced the fusion index.