| 逆转录病毒介导人VEGF在兔皮肤成纤维细胞中的表达 |
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| 引用本文： | 何天源,施娅雪,陈诗书.逆转录病毒介导人VEGF在兔皮肤成纤维细胞中的表达[J].分子细胞生物学报,2000,33(2):101-107. |
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| 作者姓名： | 何天源 施娅雪 陈诗书 |
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| 作者单位： | 何天源（上海第二医科大学人类基因治疗研究中心，上海;200025）;施娅雪（上海第二医科大学七年制学生。）;陈诗书（上海第二医科大学人类基因治疗研究中心，上海;200025） |
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| 摘 要： | 本文以人VEGFcDNA为目的基因，构建成逆转录病毒质粒载体，并进一步包装成重组缺陷型逆转录病毒，以此感染原代兔皮肤成纤维细胞，经G418筛选，获得了具有分泌VEGF能力的抗性细胞克隆，经Southern、PCR、Northern、RT-PCR等检测，证实了外源基因已整合至原代兔皮肤成纤维细胞基因组中，无重排和产生野生型病毒之患。整合的VEGF可在修饰细胞中转录，经内皮细胞增殖实验和血管通透性实验证实其表达产物具有强大的生物活性。为进一步研究VEGF的生物学功能和生理效应提供有用的工具。
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| 关 键 词： | 血管内皮生长因子 皮肤成纤维细胞 血管生成 逆转录病毒 |
| 修稿时间： | 1998-09-30 |
EXPRESSION OF VEGF ON RABBIT SKIN FIBROBLASTS USING RETROVIRAL VECTOR |
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| HE Tian Yuan,SHI Ya Xue,CHEN Shi Shu.EXPRESSION OF VEGF ON RABBIT SKIN FIBROBLASTS USING RETROVIRAL VECTOR[J].Journal of Molecular Cell Biology,2000,33(2):101-107. |
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| Authors: | HE Tian Yuan SHI Ya Xue CHEN Shi Shu |
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| Institution: | Tian Yuan
,SHI Ya Xue
,CHEN Shi Shu
;(Research Center fol Human Gene Therapy, Shanghai Second Medical University, Shanghai 200025) |
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| Abstract: | A replication-deficient recombinant retro-virus containing the cDNA coding for human vascular endothelial growth factor (VEGF) was generated, and then infected rabbit primary skin fibroblasts. After selection with G418, the transduced colonies have the ability of producing VEGF. The integration and expression of VEGF in transduced cells were confirmed by Southern
blot, PCR, Northern blot and RT-PCR assay. The VEGF secreted by transduced cells has strong bioactivity when assayed by endothelial proliferation and Miles vascular permeability assay. Thus, this study pave the way for future study of biological and physiological effect of VEGF in vivo. |
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| Keywords: | Vascular endothelial growth factor Skin flbroblasts Angeogenesis Retrovirus |
| 本文献已被 CNKI 等数据库收录！ |
