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水稻花药总蛋白质双向电泳方法的改良与优化(简报)
引用本文:文李,刘盖,王坤,彭晓珏,万翠香,李国民,陶钧,朱英国.水稻花药总蛋白质双向电泳方法的改良与优化(简报)[J].分子细胞生物学报,2006,39(5):473-476.
作者姓名:文李  刘盖  王坤  彭晓珏  万翠香  李国民  陶钧  朱英国
作者单位:武汉大学国家教育部植物发育生物学重点实验室,武汉大学国家教育部植物发育生物学重点实验室,武汉大学国家教育部植物发育生物学重点实验室,武汉大学国家教育部植物发育生物学重点实验室,武汉大学国家教育部植物发育生物学重点实验室,长沙理工大学生物与食品工程学院,长沙理工大学生物与食品工程学院,武汉大学国家教育部植物发育生物学重点实验室 武汉 430072 长沙理工大学生物与食品工程学院,长沙 410076,武汉 430072,武汉 430072,武汉 430072,武汉 430072,长沙 410076,长沙 410076,武汉 430072
基金项目:国家重大基础研究973项目(No.2001CB108806),中国高校博士点专项研究基金(20020486037)~~
摘    要:双向电泳作为蛋白质组学核心技术之一,目前已广泛地应用在植物领域,并且成功应用于水稻代谢和调节等方面的研究。谢锦云等利用溶液法提取温敏核不育水稻花药总蛋白质,利用pH3-10线性胶条分离,经银染显色后检测到约1,000个

关 键 词:水稻  花药  双向电泳
收稿时间:2006-02-10
修稿时间:2006-07-05

IMPROVEMENT OF TWO-DIMENSIONAL GEL ELECTROPHORESIS OF TOTAL PROTEINS FROM RICE ANTHERS
WEN Li,LIU Gai,WANG Kun,PENG Xiao Jue,WAN Cui Xiang,LI Guo Min,TAO Jun,ZHU Ying Guo.IMPROVEMENT OF TWO-DIMENSIONAL GEL ELECTROPHORESIS OF TOTAL PROTEINS FROM RICE ANTHERS[J].Journal of Molecular Cell Biology,2006,39(5):473-476.
Authors:WEN Li  LIU Gai  WANG Kun  PENG Xiao Jue  WAN Cui Xiang  LI Guo Min  TAO Jun  ZHU Ying Guo
Institution:Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China.
Abstract:This paper reported an improvement in 2-D gel electrophoresis of the proteome in Honglian cytoplasmic male sterile rice. An IPGphor?unit with immobile pH gradient strips was used as the first dimension and SDS-PAGE as the second. The total anther proteins were extracted using TCA/acetone and then were washed 5-6 times with acetone till the proteins were white and clean,and then tributylphosphine and DTT were added into the rehydration buffer to improve the solubility of the proteins. The 2-D gel was stained by both methods of coomassie blue G-250 and silver. Extraction of proteins,pH of the strips and rehydration of the strips were optimized and compared. Higher repeatability and better separating protein pattern could be gained by this technique.
Keywords:Rice  Anther  Two-dimensional gel electrophoresis  
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