利用氧化剂甲基紫精筛选拟南芥寿限延长突变体

在模式动物中的研究表明,寿限延长与氧化胁迫耐受能力密切有关;在模式植物拟南芥中也发现晚花突变体具有更强的抗氧化胁迫能力,但迄今为止未见有关拟南芥寿限延长突变体的研究报道。本文建立了用氧化剂甲基紫精筛选寿限延长突变体的方法,并对用该方法从经快中子诱变的拟南芥Columbia生态型M_2代群体中筛选获得的突变体SFNA-9-4进行分析,发现该突变体的抗氧化胁迫能力和寿限均显著增加。由此说明,用该方法筛选拟南芥寿限延长突变体是可行的。

SCREENING FOR LIFESPAN-EXTENSION MUTANTS WITH PARAQUAT IN ARABIDOPSIS

Genetic analyses of lifespan in model animals have revealed that extended lifespans are closely associated to increased resistance to oxidative stress. In the model plant Arabidopsis , late-flowering mutants are also found to be more tolerant to oxidative stress. However, Arabidopsis mutants with extended lifespans are poorly studied so far. In this study, a screening system for mutants with extended lifespans in Arabidopsis was established using paraquat, a potent herbicide that exacerbates O2- radical production. The relationship between lifespan and resistance to oxidative stress was examined with one of the mutants, SFNA-9-4. Compared to that of wild type, the lifespan of SFNA-9-4 is significantly extended, and its resistance to oxidative stress is also significantly elevated. These results suggest that, as in Drosophila , paraquat can also be used to screen for mutants with extended lifespans in Arabidopsis.