Fundulus heteroclitus卵子的发育能力的研究

以实验的方法来研究 Fundulus heteroclitus 卵子分裂球的发育能力,最早的工作是 Morgan 1893和1895年的报告。他在2细胞时期,用针穿破卵膜,刺死一个分裂球。针抽出后,轻轻的抑压卵膜,挤出刺死的分裂球的细胞质,但不损伤下面的卵黄。经过这样手术以后,遗留在膜内的分裂球能继续发育。68个卵子中,20个左右在胚胎形成前死亡,其余都能继续发育,成为正常的胚胎。胚胎的身体一般比正常的小,比正常的二分之一来得大。Morgan 在他1893年的论文中,认为去掉一半分裂球的卵子,发育成为大于二分之一胚胎的原因,是留存的细胞质多于二分之一的结果。因在2细胞时期,

EXPERIMENTS ON THE DEVELOPMENTAL POTENCIES OF EGG-FRAGMENTS AND ISOLATED BLASTOMERES OF FUNDULUS HETEROCLITUS

The developmental potencies of blastomeres of Fundulus heteroclitus were first studied by Morgan(1893,1895).He removed one blastomere of the egg in the 2-cell stage by puncturing it,and obtained normal embryo from the remaining blastomere.Since at this time the protoplasm had not yet entirely passed into the region of the blastoderm, the remaining egg was in reality something more than half the size of the whole,con- sequently,the resulting embryo was larger than half a normal embryo.Lewis(1912) and Hoadley...