三羟异黄酮对HER-2/neu高表达乳腺癌细胞血管生成相关因子表达的影响

三羟异黄酮(genistein)是大豆中的一种非营养成分,其结构与黄酮化合物类似,能竞争性地与雌激素受体结合,故称之为植物雌激素(phytoestrogen)。它具有广泛的生物学作用,如抗肿瘤、抗病毒、抗真菌、抗氧化、抗突变、抗高血压、抗增生等,其中genistein抑制肿瘤的血管生成是当前研究的热点之一。肿瘤的血管生成是肿瘤进一步生长转移的基础,该过程受肿瘤细胞和血管内皮细胞分泌的血管生成相关因

EFFECT OF GENISTEIN ON EXPRESSION OF ANGIOGENESIS RELATED FACTORS IN HER-2/NEU-OVEREXPRESSING BREAST CANCER CELLS

To study the molecular mechanism of inhibition of angiogenesis in HER-2/neu-overexpressing breast cancer by genistein,HER-2/neu negative expression breast cancer MCF-7cells were transfected with HER-2/neu to establish HER-2/neu-overexpressing MCF-7 cells(namedMCF-7/HER-2).Expression of vascular endothelial growth factor(VEGF),urokinase-type plasmino-gen activator(uPA),matrix metalloproteinase-2,9(MMP-2.9) in MCF-7,MCF-7/HER-2 as well asgenistein-treated MCF-7/HER-2 were measured by immunocytochemisty and Western blot.Wefound that the expression of VEGF,MMP-2,9 and uPA in MCF-7/HER-2 cells were highter thanthat in MCF-7 cells,those angiogenesis related factors expression in MCF-7/HER-2 cells signifi-cantly decreased after treatment with genistein.Genistein could inhibit expression of angiogenesis-related factors VEGF,MMP-2,9 and uPA in HER-2/neu-overexpressing breast cancer cells,andthis may be part of molecular mechanism of its anti-angiogenesis in HER-2/neu-overexpressingbreast cancer.