腺嘌呤对体外小鼠卵母细胞减数分裂的抑制

本文研究了嘌呤类物质对小鼠卵母细胞减数分裂的影响。于卵母细胞的生发泡内显微注射腺嘌呤和腺嘌呤的类似物苄基腺嘌呤可显著抑制卵母细胞的分裂的重新启动。同时发现在腺嘌呤的作用过程中,腺苷酸环化酶的激活剂氟化钠可增强其对卵母细胞的抑制作用,表明cAMP途径在小鼠卵母细胞减数分裂成熟过程中起重要作用。腺嘌呤在不同培养液中的抑制效果不一,次黄嘌呤在DMEM和EMEM中对小鼠的卵丘细胞-卵母细胞复合体(COC)和无卵丘细胞的裸卵(DO)均具有明显的抑制效应。但腺嘌呤在DMEM比在EMEM中对COC的抑制效果更强,而且腺嘌呤在DMEM中与次黄嘌呤具有协同效应,这些差别可能是由于两种培养液中不同成分如谷氨酰胺造成卵母细胞对腺嘌呤吸收差异而引起的。

THE MEIOSIS ARREST OF MOUSE OOCYTES INDUCED BY ADENINE

The effect of purine on the meiosis of mouse oocytes was studied. When ade-nine and benzyladenine, a similar substance of adenine, was microinjected into the germinal vesicle of oocytes, the meiotic maturation of oocytes could be obviously arrested depending on the dosage of injection. The stimulator of adenylate cyclase, NaF, could enhance the arrest effect of adenine. It indicated that cAMP played a important role in mouse oocytes meiotic resumption. Hypoxanthine and adenine had different effects in different medium. Hypoxanthine could obviously arrest the ma- turation of mouse cumulus oocyte complex (COC) and cumulus denuded oocytes (DO) in DMEM or EMEM. But only in DMEM adenine could obviously arrest the maturation of COC, and had co-effect with hypoxanthine in DMEM. They could almost arrest the maturation of COC and DO. Adenine in EMEM had little arrest effect on maturation of COC and DO. The different effects may result from the different adenine absorption by oocyte in DMEM and EMEM. Adding glutamine into EMEM could enhance the arrest effect of adenine to COC and DO.