离体黄瓜子叶节花芽分化与内源激素及多胺的关系

用高效液相色谱法(HPLC)测定了黄瓜子叶节花芽分化期(0-6天)内源激素及多胺的变化。结果显示,子叶培养0-2天生长素(IAA)、赤霉素(GA_3)、玉米素(ZT)、脱落酸(ABA)等4种内源激素均明显下降,4-5天略有上升,表明0-2天IAA、GA_3和ABA的剧降有利于花原基形成,3-5天较高的ZT含量有利于花器官原基的形成。除腐胺(Put)外,精胺(Spm)、亚精胺(Spd)、尸胺(Cad)在0-1天均下降,1-4天上升,4-5天再下降,Put在0-1天急剧上升,而后持续下降,表明高水平的内源多胺总量和Put可能有利于花原基分化,2天后Spm含量上升有利于花器官原基分化,而Cad含量变化可能是区别花芽和营养芽分化的特征之一。

RELATIONSHIP BETWEEN FLORAL DIFFERENTIATION AND ENDOGENOUS HORMONES AND POLYAMINES CONTENTS IN CUCUMBER COTYLEDONARY NODES CULTURED IN VITRO

Changes of endogenous hormones and polyamines(PAs) contents during floral differentiation ( 0 - 6 day) in cucumber cotyledonary nodes cultured in vitro were determined by using HPLC. The results showed that all four endogenous hormones decreased obviously within 0 - 2 day and increased slightly within 4-5 day after culture. This indicated that low levels of indole-3-acetic acid (IAA) , gibberelline(GAs) and abscisin acid (ABA) during 0 - 2 day were favorable to floral primordia differentiation, while high level of zeatin(ZT) during 3 - 5 day was favorable to floral organ primordia differentiation. Contents of spermine (Spm), spermidine(Spd) and cadavarine( Cad) were decreased within 0 - 1 day, but increased within 1 - 4 day, and then decreased again within 4 - 5 day after culture. On the contrary, content of putrescine(Put) was increased significantly on the first day and then declined during 1-6 day. Changes of PAs contents indicated that high levels of PAs and Put were favorable to floral primordia differentiation, the increasing of Spm after the second day was favorable to floral organ primordia differentiation, and change of Cad content may be one of the characteritic symbols between floral and vegetative bud differentiation.