促黄体素(LH)和人绒毛膜促性腺激素(hCG)在原索动物神经系统、哈氏窝和性腺中的分布

用抗人LH和hCG特异性抗体对原索动物神经系统、哈氏窝和性腺进行了免疫细胞化学定位。结果表明,文昌鱼脑泡和神经管中有两种不同LH-和hCG-样免疫反应阳性细胞,而皱瘤海鞘神经节对LH和hCG抗体则显免疫阴性反应。同时,首次发现在原索动物性腺(卵巢和精巢)发育早期均存在LH-和hCG-样免疫反应阳性细胞,阳性物分布在原索动物卵原细胞的胞质、核质和核仁膜以及精巢中早期生精细胞。后来,随卵巢发育和成熟,这些阳性物仍分布在卵母细胞质膜、胞质、核膜和核质上,而精巢中精细胞和精子则显免疫阴性。这些结果可为LH和hCG直接参与调节原索动物性腺发育和成熟提供形态学的新证据。

DISTRIBUTION OF LUTEINIZING HORMONE (LH) AND HUMAN CHORIONIC GONADOTROPIN (hCG) IN THE NERVOUS SYSTEM, HATSCHEK'S PIT AND GONADS OF PROTOCHORDATA

Immunocytochemical localization of the nervous system , Hatschek's pit and gonads in proto-chordata was carried out using two specific antibodies against human LH and hCG. The results indicate that there are two different kinds of LH-and hCG-like immunopositive cells in the brain vesicle and nerve tuber of amphioxus, and nerve ganglion in Styela plicata showed immurio-nega-tive reaction to LH and hCG antibodies. At the same time, we found for the first time that LH-and hCG-like immuno-positive cells existed in the early gonads (ovaries and testis) in protochor-data. Positive substance distributed in the cyto- plasm, nucleoplasm and nucleolar membrane of oogonia as well as early spermatogenic cells in testis. Afterwards, this positive substance was still distributed in the cytoplasmic membrane, cytoplasm, nuclear membrane and nucleoplasm of oocyte during the development and maturation of ovary , and spermatid and spermatozoa showed immuno-negative reaction in testis. These results will provide a new mophological proof that LH and hCG may be directly involved in regulation of the gonadal development and maturation of protochordata.