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马尾松高效再生体系的建立(简报)
引用本文:张宇,卫志明,席梦利,施季森.马尾松高效再生体系的建立(简报)[J].分子细胞生物学报,2006,39(3):271-276.
作者姓名:张宇  卫志明  席梦利  施季森
作者单位:中国科学院上海生命科学研究院植物生理生态研究所 上海200032(张宇,卫志明),南京林业大学林木遗传育种与基因工程开放实验室 南京210037(席梦利,施季森)
基金项目:国家自然科学基金项目(30170745),美洲黑杨和马尾松遗传转化研究
摘    要:利用组织培养技术快速繁殖针叶树,不仅为植树造林所需大量苗木开辟了一条快速高效的新途径,是生产优良无性系的捷径,而且也是植物基因工程技术应用于针叶树品种改良的前提条件。它将在林木种苗产业化和林木良种化进程中发挥重要作用。针叶树的离体培养和植株再生一直是植物组织培养研究中难度较大的一个领域。近几十年来,随着世界各国对发展无性系育林业的日益重视,针叶树组织培养研究取得了很大的进展1-3]。马尾松(Pinus massoniana Lamb.)是我国南方主要的造林及绿化树种,其木材和松脂具有重要的经济价值。然而在生产实践中,种子园种子…

关 键 词:马尾松  成熟合子胚  不定芽诱导  腋芽增殖  生根培养
收稿时间:2005-11-08
修稿时间:2006-03-10

EFFICIENT PLANT REGENERATION IN VITRO IN PINUS MASSONIANA L.
ZHANG Yu,WEI Zhi Ming,XI Meng Li,SHI Ji Sen.EFFICIENT PLANT REGENERATION IN VITRO IN PINUS MASSONIANA L.[J].Journal of Molecular Cell Biology,2006,39(3):271-276.
Authors:ZHANG Yu  WEI Zhi Ming  XI Meng Li  SHI Ji Sen
Institution:1Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032; 2 Laboratory of Forest Genetics and Gene Engineering, Nanjing Forestry University, Nanjing 210037
Abstract:Pinus massoniana L. is one of the important trees for afforestation in South China. The efficient system of plant regeneration from mature zygotic embryos and seedlings of masson pine was established in this study. The influences of basal media, hormones and methods for buds induction, shoots elongation and rooting were studied. The results indicate that DCR medium with 0.5 mg/L BA and 0.05 mg/L IBA shows the highest differentiation rate of adventitious buds. Induction and multiplication of axillary buds take aseptic seedlings as explants. KT has better effect than BA on the axillary buds induction. The best axillary buds induction medium is DCR medium supplemented with 1 mg/L KT and 0.2 mg/L IBA. After culturing on GD medium with 0.1 mg/L BA and 0.2 mg/L IBA for elongation,the buds were transferred on the 1/2 GD medium with 2 mg/L IBA and 0.05 mg/L BA for adventitious roots induction. Paraffin slice indicates that the adventitious buds developed from the meristematic tissue of cotyledonary epicuticula.
Keywords:Masson pine (Pinus massoniana L  )  Mature zygotic embryo  Adventitious bud induction  Multiplication of axillary bud  Rooting
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