抑郁症模型大鼠学习记忆能力变化研究

为探讨抑郁症发生发展过程中学习记忆能力的变化模式及其可能机制．分别采用21天慢性非预见性刺激法和嗅球切除法建立的抑郁症模型大鼠．运用旷场行为实验（open—field behavior）检测大鼠主动性活动能力，用Morris水迷宫法检测大鼠空间学习记忆能力，HPLC—UV法测定大鼠血清皮质醇含量。电生理法记录海马CA1区LTP与LTD，观察海马神经元的突触可塑性。结果显示：与对照组相比，两种模型的自主活动性、空间探索兴趣和学习能力都明显降低，而记忆的反馈功能没有明显的变化。同时．两种模型大鼠海马神经细胞的突触可塑性显著下降，血清皮质醇的含量则明显上升。提示两种建模方法均导致大鼠产生抑郁症状和学习能力障碍．但对记忆反馈功能无明显影响。

THE CHANGE OF LEARNING AND MEMORY ABILITY IN THE RAT MODEL OF DEPRESSION

The present study was performed to explore learning and memory ability in the depression models of rats. The chronic unpredictable stress and olfactory bulbectomy model of rats were adopted. Open-field test was used to detect the locomotion activity and HPLC-UV was employed to analyze the level of blood serum cortisol. The method of Morris water Maze was used to measure learning and memory ability and the results of LTP and LTD in hippocampus CA1 were recorded to observe the synaptic plasticity of hippocampus neurons. The results showed that compared with control group, the locomotion activity and learning ability for two models decreased extremely, while there was no apparent difference in the feedback of memory. Meanwhile, the synaptic plasticity of hippocampus neurons for two models decreased significantly and the level of serum cortisol increased evidently. These results suggested that both methods employed to build the models could cause rats depression and learning inhibition, but do no effects on the feedback of memory.