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拔节期与抽穗期玉米抗纹枯病相关QTL的初步定位
引用本文:赵茂俊,高世斌,张志明,荣廷昭,潘光堂.拔节期与抽穗期玉米抗纹枯病相关QTL的初步定位[J].分子细胞生物学报,2006,39(2):139-144.
作者姓名:赵茂俊  高世斌  张志明  荣廷昭  潘光堂
作者单位:四川农业大学玉米研究所,四川农业大学玉米研究所,四川农业大学玉米研究所,四川农业大学玉米研究所,四川农业大学玉米研究所 西南作物基因资源与遗传改良教育部重点实验室四川农业大学雅安 625014,西南作物基因资源与遗传改良教育部重点实验室四川农业大学雅安 625014,西南作物基因资源与遗传改良教育部重点实验室四川农业大学雅安 625014,西南作物基因资源与遗传改良教育部重点实验室四川农业大学雅安 625014,西南作物基因资源与遗传改良教育部重点实验室四川农业大学雅安 625014
基金项目:教育部长江学者和创新团队发展计划(IRT0453),国家科技攻关计划(2004BA525804),引进国际先进农业科学技术项目(2003—Q03)资助。
摘    要:以玉米自交系R15(抗)×478(感)的F_2分离群体为作图群体,构建了包含146个SSR标记位点的遗传连锁图谱,覆盖玉米基因组1666 cM,平均图距11.4 cM。通过麦粒嵌入法对229个F_(2:4)家系进行人工接种纹枯病菌,于玉米拔节期和抽穗期进行纹枯病的抗性鉴定。应用复合区间作图法分析两个时期的抗病QTL及遗传效应。结果共检测到17个抗性QTL,其中以拔节期病情指数为指标共检测到9个QTL,分别位于第1、2、3、4、5、6、和10染色体上,可解释的表型变异为3.72%-9.26%;以抽穗期的病情指数为指标共在7条染色体上检测到10个抗玉米纹枯病的QTL,分布于第2、3、4、5、6、8和9染色体上。单个QTL可解释的表型变异为4.27%-9.27%。两个时期共检测出2个共同QTL,它们分别位于第2染色体的bnlgl662-bnlg1940区间和第6染色体的umc1006-umc1723区间。定位结果表明两个时期检测出的抗性QTL的差异表达与玉米不同发育时期基因的时空表达有密切关系,从而反映在纹枯病的抗性位点差异性上.这为玉米抗病选育提供新的信息。

关 键 词:玉米  纹枯病  QTL定位  不同发育时期
收稿时间:2005-08-23
修稿时间:2006-01-10

INITIAL IDENTIFICATION OF QUANTITATIVE TRAIT LOCI CONTROLLING RESISTANCE TO BANDED LEAF AND SHEATH BLIGHT AT ELONGATING AND HEADING DATE IN MAIZE
ZHAO Mao Jun,GAO Shi Bin,ZHANG Zhi Ming,RONG Ting Zhao,PAN Guang Tang.INITIAL IDENTIFICATION OF QUANTITATIVE TRAIT LOCI CONTROLLING RESISTANCE TO BANDED LEAF AND SHEATH BLIGHT AT ELONGATING AND HEADING DATE IN MAIZE[J].Journal of Molecular Cell Biology,2006,39(2):139-144.
Authors:ZHAO Mao Jun  GAO Shi Bin  ZHANG Zhi Ming  RONG Ting Zhao  PAN Guang Tang
Institution:The Maize Research Institute, Ministry of Education, Sichuan Agriculture University, Ya'an.
Abstract:The genetic linkage map was constructed with 146 SSR markers based on a maize population consisting of 229 F2 individuals from the cross R15 (resistant) x 478 (susceptible), covering 1666cM on a total of ten chromosomes, with an average interval length of 11.4 cM. The disease index from the population of 229 F24 lines were evaluated for BLSB resistance under artificial inoculation at elongating stage and heading date. With the method of composite interval mapping (CIM) described in QTL cartographer v2.0 procedure, 9 QTLs among of 17 QTLs were identified on 1, 2, 3, 4, 5, 6 and 10 chromosomes at Jointing Stage, accounting for 3.72% to 9.26% of the phenotypic variance. The other 10 QTLs were identified on 2, 3, 4, 5, 6, 8 and 9 chromosomes at elongating stage and heading date, accounting for 4.27% to 9.27% of the phenotypic variance. Among of them, two QTLs were detected at two stages, which were located between bnlgl600-umc1818 and umc1006-umc1723 on chromosome 2 and 6, respectively. The result indicated that the significant difference about QTL Controlling Resistance between the two stages had a close connection with Developing Stages, which was showed on the resistance of banded leaf and sheath blight in maize. So this result provided new information to the resistance breeding of maize.
Keywords:Maize  BLSB  QTL  Different  Developing stages
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