| 水通道蛋白1-C末端肽段/GST融合蛋白的原核表达(简报) |
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| 引用本文： | 李晓萌,李江,杨南扬,关新刚,张淑芝,惠伟丽,麻彤辉.水通道蛋白1-C末端肽段/GST融合蛋白的原核表达(简报)[J].分子细胞生物学报,2008,41(1):81-85. |
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| 作者姓名： | 李晓萌 李江 杨南扬 关新刚 张淑芝 惠伟丽 麻彤辉 |
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| 作者单位： | [1]东北师范大学生命科学学院膜通道实验室,长春130024 [2]吉林大学口腔医院,长春130041 |
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| 基金项目： | 东北师范大学校科研和教改项目 |
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| 摘 要： | 水通道蛋白(Aquaporin,AQP)是一类选择性高效转运水分子的细胞膜通道蛋白,广泛存在于原核和真核生物细胞的细胞膜上,主要介导自由水分子的被动跨膜转运,对保持细胞内外液环境的稳态平衡起着重要的作用.
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| 关 键 词： | AQP1 GST融合蛋白 E.coli 水通道蛋白 肽段 融合蛋白 原核表达 简报 COLI FUSION PROTEIN DOMAIN TERMINAL GLUTATHIONE 作用 稳态平衡 环境 外液 胞内 跨膜转运 膜上 真核生物细胞 存在 细胞膜 |
| 收稿时间： | 2007-07-02 |
| 修稿时间： | 2007-11-25 |
EXPRESSION OF GLUTATHIONE S-TRANSFERASE-AQUAPORIN 1 CARBOXYL TERMINAL DOMAIN FUSION PROTEIN IN ESCHERCHIA COLI |
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| LI Xiao Meng,LI Jiang,YANG Nan Yang,GUAN Xin Gang,ZHANG Shu Zhi,HUI Wei Li,MA Tong Hui.EXPRESSION OF GLUTATHIONE S-TRANSFERASE-AQUAPORIN 1 CARBOXYL TERMINAL DOMAIN FUSION PROTEIN IN ESCHERCHIA COLI[J].Journal of Molecular Cell Biology,2008,41(1):81-85. |
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| Authors: | LI Xiao Meng LI Jiang YANG Nan Yang GUAN Xin Gang ZHANG Shu Zhi HUI Wei Li MA Tong Hui |
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| Institution: | Membrane Channel Research Laboratory, School of Life Sciences, Northeast Normal University, Changchun 130024. lixm441@nenu.edu.cn |
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| Abstract: | We constructed a recombinant plasmid of water channel protein Aquaporin 1 (AQP1) carboxyl terminal domain (DNA sequence from 700bp-801bp) in pGEX-4T-1 vector and express the carboxyl terminal hydrophilic peptide AQP1 in E. coli. In this study, the DNA sequence of AQP1 hydrophilic peptide was amplified by PCR and was cloned into pGEX-4T-1 expression vector. After identified by restriction enzyme digestion and sequencing, the recombinant clone was transformed into the competent expression cells of E. coli BL21. The GST-AQP1 fusion protein was induced by IPTG and further purified by Glutathione Sepharose 4B to obtain a fusion protein with molecular weight of 30KD. So the fusion protein of AQP1 C-terminal hydrophilic peptide combined with GST was successfully expressed and purified. We set up important bases for the further research in AQP1 gene function. |
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| Keywords: | Aquaporin 1 GST fusion protein E coil |
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