| 用ISSR标记技术分析山药品种遗传多样性 |
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| 引用本文： | 周延清,景建洲,李振勇,浩健,贾敬芬,张宝华,郝建国.用ISSR标记技术分析山药品种遗传多样性[J].分子细胞生物学报,2005,38(4):324-330. |
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| 作者姓名： | 周延清 景建洲 李振勇 浩健 贾敬芬 张宝华 郝建国 |
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| 作者单位： | 西北大学生命科学学院，华美生物工程公司博士后工作站，华美生物工程公司博士后工作站，华美生物工程公司博士后工作站，西北大学生命科学学院，温县农业科学研究所，西北大学生命科学学院 西安 710069 河南师范大学生命科学学院，新乡 453007，洛阳 471003 清华大学生物科学与技术系，北京 100084，洛阳 471003，洛阳 471003，西安 710069，河南温县 454881，西安 710069 |
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| 基金项目： | 国家自然科学基金（30370697） |
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| 摘 要： | 利用ISSR标记技术对28个山药品种的遗传多样性进行分析。结果表明,从44条ISSR引物中可筛选出7条能够扩增出清晰、稳定条带的引物;这7条ISSR引物对28个山药品种扩增条带间存在较大差异,多态性条带比率为83.01%,Shannon多样性指数为0.3191;构建的分子树状图将28个山药品种划分为4组:第一组含有日本白、花山药和日本园3个品种;第二组为小叶山药;第三组为嵩野1号;其余23个品种归入第四组。而且主成分分析结果支持上述的聚类分析结果。这为利用ISSR标记技术鉴定山药品种,为有效地利用山药种质资源提供了依据。
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| 关 键 词： | 山药 ISSR 品种鉴定 遗传多样性 |
| 修稿时间： | 2004年11月29 |
GENETIC DIVERSITY OF YAM (DIOSCOREA OPPOSITA THUNB) DETECTED BY ISSR MARKERS |
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| ZHOU Yan Qing,JING Jian Zhou,LI Zhen Yong,HAO Jian,JIA Jing Fen,ZHANG Bao Hu,HAO Jian Guo College of Life Sciences,Northwest University,Xi'an ,China Postdoctoral Programme,Sino-American Biotechnology Company,Luoyang ,China.GENETIC DIVERSITY OF YAM (DIOSCOREA OPPOSITA THUNB) DETECTED BY ISSR MARKERS[J].Journal of Molecular Cell Biology,2005,38(4):324-330. |
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| Authors: | ZHOU Yan Qing JING Jian Zhou LI Zhen Yong HAO Jian JIA Jing Fen ZHANG Bao Hu HAO Jian Guo College of Life Sciences Northwest University Xi'an China Postdoctoral Programme Sino-American Biotechnology Company Luoyang China |
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| Institution: | ZHOU Yan Qing,JING Jian Zhou,LI Zhen Yong,HAO Jian,JIA Jing Fen,ZHANG Bao Hu,HAO Jian Guo College of Life Sciences,Northwest University,Xi'an 710069,China Postdoctoral Programme,Sino-American Biotechnology Company,Luoyang 471003,China Department of Biological and Biotechnology,Tsinghua University,Beijing 100084,China College of Life Sciences,Henan Normal University,Xinxiang 453007,China Wenxian Institute of Agricultural Sciences,Wenxian 454881,China |
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| Abstract: | Genetic diversity of 28 cultivars of yam (Dioscorea opposita Thunb) was assessed by means of Inter-simple sequence repeat (ISSR) markers. The results showed that seven proper primers, with rich polymorphism, could be selected from a total of forty four ISSR ones; distinct differences appeared among 28 cultivars amplified bands, and the rate of polymorphic bands was 83.01%;Shannon's Information index was 0.3191; a Jaccard's genetic similarity matrix and a den- drogram for these cultivars were formed, in which they could be divided into four groups: Groupl was composed of D.opposita.cv. Ribenbai, D.opposita.cv.Huashanyao and D.opposita.cv.Ribenyuan; Group2 contained D.opposita.cv. Xiaoye; Group3 contained D.opposita.cv. No.1 Songye; other 23 cultivars were put into Group4. PCA(Principal component analysis) was employed to evaluate the resolving power of the markers to differentiate among them. This laid the foundation of the identi- fication of yam cultivars and the efficient use of its germplasm resources. |
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| Keywords: | Dioscorea opposita Thunb ISSR marker Cultivar identification Genetic diversity |
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