甘草腺毛的形态发生和组织化学研究

利用扫描电镜及薄切片技术对甘草的腺毛形态发生和发育过程进行了观察，并对腺毛发育过程中黄酮类成分积累进行了组织化学定位研究。结果表明：甘草腺毛为多细胞构成的盾状腺毛，有长柄和短柄2种类型；前者主要分布在花萼片上，而后者主要分布于叶片上。组化鉴定结果显示：腺毛中存在着黄酮类成分、其他亲脂类和非纤维素多糖类成分；在腺毛的发育过程中，黄酮类物质是随腺毛的发育成熟，在头部盘状结构的分泌细胞及角质层下腔中积累。研究结果对进一步探讨甘草叶中黄酮类成分的合成及其作用提供科学依据。

MORPHOGENESIS AND HISTOCHEMICAL INVESTIGATION OF PELTATE GLANDULAR TRICHOMES ON GLYCYRRHIZA URALENSIS FISCH. LEAVES

Anatomical and histochemical investigation of the glandular trichomes on the leaves of Glycyrrhiza uralensis Fisch.were carried out by using scanning electronic microscopy (SEM), Light microscopy and fluorescence microscopy. Two types of peltate glandular trichomes can be distinguished: one occurred on the leaves abaxial and adaxial surface with a short stalk and the second on the calyx surface with a long stalk. Secretory trichomes started as outgrowth of epidermal cells, subsequent divisions gave rise to trichomes made up of a basal cell, stalk cells and a multicellular secretory head. The histochemical identification revealed that complex substance excreted by the secretory cells contained flavoniods, polysaccharides and lipophilic compounds. During the development of glandular trichomes, only mature trichomes with ten-celled secretory head or even more cells could be observed the flavonoids inside or in subcuticular space but before that stages the flavonoids were not found. The findings provided a scientific basis for understanding the flavonoids synthesis and biological function.