小鼠胚泡黏附时子宫内膜nm23-M1/NDPK A的表达

nm23家族除与肿瘤转移抑制有关,它还参与调节正常细胞的发育、增殖、分化及凋亡等过程。运用RT-PCR、Western blot 和免疫组织化学技术,分析小鼠胚泡黏附时子宫内膜着床点和着床旁组织nm23-M1/NDPK A 的表达,以未交配鼠作对照,为进一步阐明胚泡着床的机制提供有意义的实验依据。RT-PCR 结果显示,小鼠胚泡黏附时子宫内膜nm23-M1/NDPK A mRNA 表达明显高于对照组,并且着床点明显高于着床旁,Western blot 和免疫组织化学分析nm23-M1/NDPK A 蛋白表达,也得到一致的结果。提示nm23-M1/NDPK A 参与胚泡着床这一重要生命活动过程。

STUDY ON THE EXPRESSION OF nm23-M1/NDPK A DURING THE BLASTOCYST ADHESIVENESS IN THE MOUSE ENDOMERIUM

The nm23 gene was originally identified as a metastasis suppressor gene by differ-ential hybridization of non-metastatic and metastatic clones of murine K-1735 melanoma cell lines.Subsequently,other highly homologous genes were characterized and shown to code for nucleosidediphosphate kinase (NDPK) isotypes.These enzymes have been evidenced in a wide variety of or-ganisms.In human,up to eight distinct but highly related genes have been discovered.Thenm23-H1 and nm23-H2 gene products share 88% identity and are about 95% and 98% identicalto the murine nm23-M1 and nm23-M2 proteins respectively.A number of data indicate that nm23family,initially documented as metastasis suppressors in some cancer types,are involved in thecontrol of normal development,differentiation and apoptosis.The trophoblast cells of blastocyst and of the normal first-trimester placenta share some phe-notypic similarities with malignant cells,such as rapid proliferation and ability to invade neigh-bouring tissues,including basement membrane,during the process of implantation and placentaldevelopment,but do not have the ability for unlimited growth or metastasis,as few trophoblastcells can be identified in the deciduas,and trophoblasts rarely penetrate the maternal blood ves-sels.Although some data indicate that nm23 family play an important role in regulation of repro-ductive processes,little is know about its actual mechanism.In our studies,we performed RT-PCR,Western blot and immunohistochemcal analyses of nm23-M1/NDPK A expression during theblastocyst adhesiveness in the mouse endometrium.The nm23-M1 mRNA expression was shownprogressive increase in D_5 mice endometrium by RT-PCR,which was consistent with the resultobtained by Western blot and immunohistochemistry.It is suggested that nm23-M1/NDPK A wasinvolved in the process of blastocyst implantation.