金鱼精子质膜和核膜的区域特异性

本文结合采用扫描和透射电子显微镜(包括冷冻断裂-蚀刻、超薄切片以及细胞化学染色法),研究了金鱼精子的超微结构特征,结果表明金鱼精子的质膜和核膜都具有区域特异性:1)精子质膜内大部分区域含有许多蛋白颗粒,但在特定区域内,蛋白颗粒呈有序排列,构成晶格状结构。2)精子头颈部和尾部均有液泡密集之处,凡是覆盖着液泡区的质膜内,几乎都不含有蛋白颗粒。3)液泡区能被细胞化学方法染成致密色,表明内含糖蛋白。4)核膜孔只集中存在于靠近颈部的核膜上,而其他部分则没有。本文对上述诸点进行了讨论。

REGIONAL SPECIFICITY WITHIN PLASMA MEMBRANE AND NUCLEAR MEMBRANE OF GOLDFISH SPERM

Combincd SEM and TEM technique including thin sectioning, freeze fracture and etching as well as cytochemical staining have been used for ultrastructral study on goldfish (Carassius auratus) sperm. It has been shown that primitive sperm plasma membrane and nuclear membrane are differentiated with regional specificity. The results from this study can be summerized as follows:1. Intercalated protein particles are highly organized in the plasma membrane in the certain region of the head to form crystalline-like structure, in contrast rest of the area is rich in randomly or clustered particles.2. Many vesicles in different size are often tightly packed in the head, neck and tail regions. Only these plasma membrane covering the vesicles contain almost no protein particles.3. The vesicles can be densely stained cytochemically, suggesting the existence of glycoprotein.4. Most of the nuclear membrane have no nuclear pores on it except the area near the neck part where many nuclear pores concentrate.