注射装载孕酮的红细胞膜泡诱发蟾蜍卵成熟的研究

中华大蟾蜍长足的卵母细胞,经注入装载水相孕酮的红细胞膜泡,能被诱发成熟;直接注入水相孕酮的卵母细胞,无能恢复成熟分裂。将蛋白酶处理的红细胞制备成装载水相孕酮的膜泡,注入卵内,照样能诱发其成熟分裂;然而,分别用根皮素结合或磷脂酶A_2水解红细胞膜磷脂,制备的膜泡,虽亦包裹着水相孕酮,但注射的卵母细胞都未能被诱发成熟。这些结果表明,在通过红细胞膜转运孕酮诱发卵母细胞成熟过程中,红细胞膜上的某些膜蛋白可能不是必要的成份,而膜磷脂类却是关键成份,它不仅可能保证孕酮不被迅速代谢,且保证孕酮从卵母细胞内部诱发成熟分裂。

STUDIES ON TOAD OOCYTES MATURATION INDUCED BY MICROINJECTION OF PROGESTERONE-LOADED HUMAN RED BLOOD CELL GHOSTS

It was found that the meiotic division of full-grown oocytes of Bufo bufo gargari-zans could be induced by microinjection of aqueous progesterone-loaded human red blood cell ghosts (Fig,1,A).Neither the aqueous solution of progesterone nor the red blood cell ghost itself could induce the resumption of meiotic division,when they were microinjected into oocytes respectively (Fig.1,B,C and D).The effects on the induction of oocyte meiotic division of chemical or enzymatic pre-treatment of the red blood cell membrane were analysed:no meiotic division could be resu- med by microinjecting the progesterone-loaded ghosts whose membrane had been pretreated with phloretin (200 !M) (Fig.2,A) or pho-spholipase A_2 (Fig.3,C); but the meiotic di-vision still can be induced by microinjecting the progesterone-loaded ghosts pretreated with pronase E (Fig.3,B).All these results in-dicated above reveal that phospholipids of me-mbrane system are necessary for progesterone to avoid any enzymatic modification in the oocyte and to realize its normal transmem-brane signal in the resumption course of meio-tic division.