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莴苣助细胞发育过程中钙的分布研究
引用本文:邱义兰,刘如石,田惠桥.莴苣助细胞发育过程中钙的分布研究[J].分子细胞生物学报,2007,40(4):253-261.
作者姓名:邱义兰  刘如石  田惠桥
作者单位:湖南师范大学生命科学学院,湖南师范大学生命科学学院,厦门大学生命科学学院 长沙 410018 厦门大学生命科学学院,厦门 361005,长沙 410018 厦门大学生命科学学院,厦门 361005,厦门 361005
摘    要:用焦锑酸盐沉淀法对莴苣助细胞中的钙分布进行了观察。结果表明,开花前3天刚形成的助细胞中的钙颗粒很少:开花前2天助细胞壁中的钙颗粒增加;开花前1天助细胞珠孔端细胞壁加厚,其中积累了许多钙颗粒:开花当天助细胞珠孔端的丝状器中聚集了大量的钙颗粒。授粉后1h时两个助细胞的结构和钙分布发生差异,一个呈退化状,其中的钙颗粒明显增多,另一宿存助细胞中的钙分布与授粉前相似。去雄不授粉1天后两个助细胞均保持完好,且两助细胞中的钙分布没有明显差异,表明由花粉管引起一个助细胞中钙含量增加进而导致了助细胞退化。退化助细胞在卵细胞与中央细胞之间形成一薄层。助细胞退化后不同部位的钙颗粒呈现出与受精作用密切有关的变化:授粉后1h时,钙主要聚集在近合点端部位;授粉后2.5h卵细胞即将受精,这时许多细小的钙颗粒主要聚集在卵细胞与中央细胞之间的薄层中;授粉后4h精、卵细胞已融合,这时退化助细胞合点端的钙颗粒明显减少,而在其珠孔端又聚集了较多的钙。上述助细胞中的钙含量变化与吸引花粉管进入胚囊和促使精卵细胞融合密切有关。

关 键 词:莴苣  助细胞  
修稿时间:2007-02-012007-05-10

STUDIES ON THE CALCIUM DISTRIBUTION IN DEVELOPING SYNERGIDS OF LETTUCE (Lactuca sativa L.)
QIU Yi Lan,LIU Ru Shi,TIAN Hui Qiao.STUDIES ON THE CALCIUM DISTRIBUTION IN DEVELOPING SYNERGIDS OF LETTUCE (Lactuca sativa L.)[J].Journal of Molecular Cell Biology,2007,40(4):253-261.
Authors:QIU Yi Lan  LIU Ru Shi  TIAN Hui Qiao
Institution:1.School of Life Sciences, Hunan Normal University, Changsha 410081;2.School of Life Sciences, Xiamen University, Xiamen 361005
Abstract:Potassium antimonite was used to locate calcium in the synergids of lettuce (Lactuca sativa L) during their development.The two synergids on 3d before anthesis formed evident polarity with most cytoplasm located in the micropylar end and nucleus in the middle and a big vacuole in the chalazal end.At this time,calcium precipitates were a few in both cells.Calcium precipitates in the two synergids began to increase on 2d before anthesis.Synergid wall in the micropylar end thickened on ld before anthesis,in which many calcium precipitates located.Near anthesis,synergids formed filiform apparatus in which abundant calcium precipitates accumulated to prepare for attract- ing pollen tubes entering.At anthesis,the distribution of calcium precipitates between two synergids was the same.At 1h after pollination,calcium precipitates evidently increased in one synergid that seemed to degenerate,the other one was persistent and the distribution of calcium granules did not change.Two synergids kept intact at 1d after emasculated,and the distribution of calcium precipi- tates did not display difference,suggesting that the degeneration of one synergid was caused by ap- proaching pollen tubes which might give some signal to induce calcium increase of the synergid.Be- fore fusion of sperm cell with egg cell,the cytoplasm of degenerated synergid embraced the egg and formed a thin layer between the egg and the central cell.Calcium precipitates in the different parts of degenerated synergid were closely connected with the fertilization:calcium precipitates accumulat- ed in the near ehalazal end of degenerated synergid at 1h after pollination.At 2.5h after pollination, the calcium precipitates increased at the chalazal end,especially abundant in the thin layer between the egg and the central cell.However,at 4h after pollination,the fertilization had finished at this time,the distribution of calcium precipitates in degenerated synergid changed again:the precipitates decreased at the chalazal end and increased at the micropylar end.The above-mentioned results sug- gested that calcium in the degenerated synergid played an important role during lettuce fertilization.
Keywords:Lettuce  Synergid  Calcium
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