| 分子佐剂C3d上调Raji细胞协同刺激分子B7-1和B7-2的表达(简报) |
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| 引用本文： | 余敏,李大金,王秀丽,袁敏敏,朱影,姚晓英,李华萍. 分子佐剂C3d上调Raji细胞协同刺激分子B7-1和B7-2的表达(简报)[J]. 分子细胞生物学报, 2006, 39(1): 77-82 |
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| 作者姓名： | 余敏 李大金 王秀丽 袁敏敏 朱影 姚晓英 李华萍 |
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| 作者单位： | 复旦大学附属妇产科研究所生殖免疫室，复旦大学附属妇产科研究所生殖免疫室，复旦大学附属妇产科研究所生殖免疫室，复旦大学附属妇产科研究所生殖免疫室，复旦大学附属妇产科研究所生殖免疫室，复旦大学附属妇产科研究所生殖免疫室，复旦大学附属妇产科研究所生殖免疫室 上海 200011，上海 200011，上海 200011，上海 200011，上海 200011，上海 200011，上海 200011 |
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| 基金项目： | 国家自然科学基金(30271235)上海市自然科学基金(00ZB14058)教育部博士学科点专项科研基金(9941)卫生部优秀青年人才专项科研基金(97031)资助。 |
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| 摘 要： | 我们在以往研究中,引入选择性增强体液免疫效应的新型分子佐剂C3d,成功构建了重组避孕疫苗hCGB-C3d3,通过免疫Th2型优势的 BALB/c小鼠和,Th1型优势的C57BL/6小鼠,显示分子佐剂C3d在不同品系小鼠均使免疫效应从Th1型细胞免疫向Th2型体液免疫偏倚。
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| 关 键 词： | 分子佐剂C3d hCGβ B7-1 B7-2 |
| 收稿时间： | 2005-05-16 |
| 修稿时间： | 2005-10-30 |
MOLECULAR ADJUVANT C3d UP-REGULATES BOTH B7-1 AND B7-2 EXPRESSION ON Raji CELLS |
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| YU Min,LI Da Jin,WANG Xiu Li,YUAN Min Min,ZHU Ying,YAO Xiao Ying,LI Hua Ping. MOLECULAR ADJUVANT C3d UP-REGULATES BOTH B7-1 AND B7-2 EXPRESSION ON Raji CELLS[J]. Journal of Molecular Cell Biology, 2006, 39(1): 77-82 |
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| Authors: | YU Min LI Da Jin WANG Xiu Li YUAN Min Min ZHU Ying YAO Xiao Ying LI Hua Ping |
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| Affiliation: | Laboratory of Reproductive Immunology, Institute of Obstetrics and Gynecology, Fudan University, Shanghai 200011. |
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| Abstract: | To explore modulation of the molecular adjuvant C3d in the hCGbeta -C3d3 fusion protein in costimulatory molecule expression on Raji cells by linking to the surface molecule CD21. Raji cells, B cell line, were incubated with the purified hCGbeta-C3d3, hCGbeta or C3d3 protein for 15 hours respectively in the interference of anti-CD21 monoclonal antibody or not. The culture concentration of each group was 10, 30, or 90 microg/ml respectively. The expression of both B7-1 and B7-2 on Raji cells were analyzed by flow cytometric assay. It was observed that compared to hCGbeta or C3d3 protein, hCGbeta-C3d3 up-regulated both B7-1 and B7-2 expression on the Raji cells, and the effect was in a dose-dependent manner. The up-regulation effect was blocked efficiently by anti-CD21 mAb. The results showed that molecular adjuvant C3d may up-regulate both B7-1 and B7-2 expression on Raji cell via C3d/CD21/CD19 complex, thus enhance the antigen presentation of B cell, and the interaction between B cell and T cell. |
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| Keywords: | B7-1 B7-2 |
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