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大豆类受体蛋白激酶基因GmRLCK的克隆及初步分析
引用本文:李鹏丽,马媛媛,甘睿,张丽文,李小平,王勇,王宁宁.大豆类受体蛋白激酶基因GmRLCK的克隆及初步分析[J].分子细胞生物学报,2006,39(3):208-216.
作者姓名:李鹏丽  马媛媛  甘睿  张丽文  李小平  王勇  王宁宁
作者单位:南开大学植物生物学与生态学系 天津300071
基金项目:国家自然科学基金资助项目(NSFCNo.30270140)资助~~
摘    要:植物类受体蛋白激酶(receptor-likeproteinkinase,RLK)在高等植物生长发育和环境刺激的信号传导中起着重要的作用。本文报告了一个新的大豆类受体蛋白激酶基因的全长cDNA克隆及对其基因结构和功能的初步分析。研究表明该基因序列编码的蛋白包含一个跨膜域、一个具有丝氨酸/苏氨酸激酶活性的胞内域和一个缺少N-末端信号肽的胞外域。采用生物信息学方法分析表明,该基因与一些拟南芥菜类受体蛋白激酶基因具有很高的相似性,这些激酶N-末端都缺少信号序列,属于植物胞质类受体激酶(receptor-likecytoplasmickinase,RLCK)亚家族。因此命名该大豆基因为GmRLCK(GenBankAccessionNo.AY687390)。对GmRLCK激酶域中磷酸化可能性较高的位点进行了预测。RT-PCR的结果表明,GmRLCK在大豆子叶、根、花以及豆荚中都有较高的表达,而在胚根、茎和成熟叶片中的表达相对较弱。进化分析表明GmRLCK与一些衰老相关的植物类受体蛋白激酶具有较近的亲缘关系。

关 键 词:大豆  类受体蛋白激酶  胞质类受体激酶  结构预测  反转录PCR  进化分析
收稿时间:2005-08-24
修稿时间:2006-03-10

CLONING AND CHARACTERIZATION OF A NOVEL SOYBEAN RECEPTOR-LIKE PROTEIN KINASE GENE GmRLCK
LI Peng Li,MA Yuan Yuan,GAN Rui,ZHANG Li Wen,LI Xiao Ping,WANG Yong,WANG Ning Ning.CLONING AND CHARACTERIZATION OF A NOVEL SOYBEAN RECEPTOR-LIKE PROTEIN KINASE GENE GmRLCK[J].Journal of Molecular Cell Biology,2006,39(3):208-216.
Authors:LI Peng Li  MA Yuan Yuan  GAN Rui  ZHANG Li Wen  LI Xiao Ping  WANG Yong  WANG Ning Ning
Institution:Department of Plant Biology and Ecology, Nankai University, Tianjin 300071
Abstract:Protein kinases play key roles in transduction of both environmental and developmental signals in higher plants. Here reported full-length cDNA cloning and preliminary structural and functional analysis of a novel soybean receptor-like protein kinase (RLK) gene (GenBank Accession No.AY687390). The deduced protein product of this gene contains a transmembrane region,an intracellular catalytic domain possessing serine/threonine kinase activity,and an extracellular domain which lacks the N-terminus signal peptide. Bioinformatic analyses revealed highly similarity between this soybean gene and a group of the Arabidopsis RLK genes which all lack of N-terminus signal sequence and belong to RLCK subfamily. Thus,it was designated GmRLCK. Some residues of GmRLCK that showed high probability to be phosphorylated were also predicted. RT-PCR analysis revealed higher expression levels of GmRLCK in soybean cotyledons,roots,flowers and young pods;whereas lower expression levels were detected in radicles,stems and mature leaves at vegetative stage. Phylogeny analysis suggested that GmRLCK was more close to and might have a common ancestor with several senescence-associated RLKs.
Keywords:Soybean  RLKs  RLCK  Structure prediction  RT-PCR  Phylogeny analysis
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