不同冷冻保护剂对猪前体脂肪细胞冷冻后细胞活力的影响(简报)

与实验动物鼠相比,猪的生物学特性与人更为接近1],肥胖程度更高,因而猪前体脂肪细胞更适合用来研究肥胖及其相关疾病,但猪原代前体脂肪细胞生长周期较长,且在常规培养条件下同一批次的细胞很难长期保存,而猪作为实验动物的价格又相对昂贵,如果采取适当的方法将猪前体脂肪细胞在超低温条件下保存,使其生命活动固定在某一阶段而不衰老死亡2],既可节省实验经费,又能保证研究的可靠性与连续性。因而建立一种较为理想的猪前体脂肪细胞冻存方法具有重要的实际意义。目前,国内外尚未见到冷冻保存猪前体脂肪细胞的报道。本实验以猪前体脂肪细胞为…

EFFECTS OF DIFFERENT CRYOPROTECTANTS ON POST-THAW VIABILITY OF PORCINE PREADIPOCYTE

To explore the optimal cryoprotectant for porcine preadipocyte cryppreservation,the primary pig preadipocytes were cryopreseved with DMSO, ethlyleneglycol(EG),PVP and DMSO PVP cryoprotective agents (CPA) and without CPA,respectively. After 30d, the cells were recovered, viability of pig preadipocyte cryopreserved with different CPAs was evaluated by the Typlan exclusion, growth characteristic was analyzed with MTT, the differentiation potentials of pig preadipocytes were observed by morphology change from preadipocyte to mature adipocyte and Oil Red O staining. The results were as follows: four cryoprotectants all could protect the pig preadipocyte from frozen at a certain degree, and the efficiency was as follows: PVP>DMSO PVP>DMSO>EG>without cryoprotectant. The growth state and the lipogenic capability of the pig preadipocytes cryopreserved with PVP were similar to those unfreezing cells. Thus, we draw a conclusion: PVP is the optimal cryoprotectant for pig preadipocyte.