粉防己碱对豚鼠链霉素性肾损伤的干预作用

研究粉防己碱(Tetrandrine,Tet)对链霉素(Streptomycin,SM)所致的急性肾损伤的干预作用及其保护机制。将双耳ABR阈值≤5dB健康白色红目豚鼠分为(1)正常对照组、(2)Tet组、(3)SM组、(4)SM Tet组,于第11天留取尿标本和肾组织,用浊度法测定尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)活性,用光镜、电镜观察肾组织学改变。形态学检测显示SM Tet组肾脏病理变化比SM组小,凋亡细胞也明显少于SM组。SM Tet组NAG活性比SM组低(P<0.01)。上述结果表明粉防己碱对链霉素所致的急性肾毒性效应有明显的拮抗作用,在药物性器官损伤防护中具有潜在的应用前景。

THE INTERVENTION OF TETRANDRINE IN THE ACUTE RENAL INJURY INDUCED BY STREPTOMYCIN SULFATE IN PUINEA PIGS

The article investigated the intervention and the protective mechanism of tetrandrine (Tet) on acute renal injury induced by streptomycin (SM). The experimental healthy albinoa pigs of each sex with both ears' threshold of auditory brainstem response (ABR) < or = 5dB were divided into 4 groups by randomized blocks, including group 1 (control), group 2 (tetradrine), group 3 (SM) and group 4(Tet+SM). After 10 days, the urinary specimen were set apart for the determination of NAG activity, renal specimen were reserved for observing the renal histology. The renal histology shows that the injury of group 3 is most grievous, the injury of group 4 is lighter than that of group 3, in addition, the apoptosis is obviously less than that of group 3. The activity of urine of NAG in group 4 is lower than that of group 3 (P < 0.01). The results suggested that tetrandrine has obvious antagonism action upon renal toxic effect induced by SM. Therefore, the tetrandrine has potential application prospect in protection against pharmaceutical organ injury.