Lack of orthogonal particle assemblies and presence of tight junctions in astrocytes of the goldfish (Carassius auratus) |
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| Authors: | Wolburg Hartwig Kästner Roland Kurz-Isler Gertrud |
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| Institution: | (1) Laboratoire de Physiologie comparée, ERA 955 du CNRS  Neurobiologie du Développement et Endocrinologie , Université des Sciences et Techniques du Languedoc, Montpellier, France;(2) Unité de Recherches sur le Métabolisme Hydro-minéral, INSERM U 120, Le Vésinet, France;(3) Department of Physiology, University of British Columbia, Vancouver, Canada;(4) Laboratoire de Physiologie comparée, Université des Sciences et Techniques du Languedoc, 34060 Montpellier Cedex, France |
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| Abstract: | Summary Specific antibodies raised against a human 28 000 dalton cerebellar calcium-binding protein (CaBP) were used in an immunocytochemical study during development of the rat cerebellum. Both light and electron microscopy showed (1) that labelling was entirely restricted to the Purkinje cells, (2) that it appeared very early in Purkinje cell development, (3) that the entire cell was labelled from the tip of the smallest dendrites to the axonal terminals, and (4) that with increasing age, the immunoreaction appeared to be progressively restricted to the cell and organelle membranes. |
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| Keywords: | Calcium-binding protein (CaBP) Immunocytochemistry Rat cerebellum Development Vitamin D |
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