Occurrence of immunoreactive enkephalins in a neurohemal organ and other nervous structures in the eyestalk of the shore crab,Carcinus maenas L. (Crustacea,Decapoda) |
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| Authors: | Peter P Jaros Heinrich Dircksen Dr Rainer Keller |
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| Institution: | (1) Present address: FB Biologie der Universität Oldenburg, Postfach 2503, 2900 Oldenburg, Federal Republic of Germany;(2) Rheinische Friedrich-Wilhelms-Universität, Institut für Zoophysiologie, Bonn, Bundesrepublik Deutschland;(3) Universität Bonn, Institut für Zoophysiologie, Endenicher Allee 11-13, 5300 Bonn, Federal Republic of Germany |
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| Abstract: | Summary Light-microscopical observations with immunofluorescence and peroxidase staining procedures revealed leu-enkephalin-like immunoreactivity in axon profiles of the sinus gland (SG) and in single small neurons in the optic ganglia of the eyestalk of Carcinus maenas. Electron microscopy of the SG showed reactivity to be associated with neurosecretory granules 82±23 nm in diameter. High performance liquid chromatography of SG-extracts revealed radioimmunoreactive substances with the retention times of synthetic met- and leu-enkephalin and met-enkephalin-Arg6-Phe7, respectively. |
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| Keywords: | Immunocytochemistry Enkephalins Neurohemal organ Carcinus maenas |
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