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Isolation,identification and quantitative evaluation of specific cell types from the mammalian gastric mucosa
Authors:M Beinborn  J Giebel  M Linck  Y Cetin  M Schwenk  K -F Sewing
Institution:(1) Department of General Pharmacology, Medical School, Konstanty-Gutschow-Str. 8, D-30625 Hannover, Germany;(2) Department of Anatomy, Medical School, Konstanty-Gutschow-Strasse 8, D-30625 Hannover, Germany;(3) Present address: Gastroenterology Division, Tufts University/New England Medical Center, 750 Washington Street, 02111 Boston, MA, USA;(4) Present address: Institute of Anatomy, Ernst-Moritz-Arndt-University, Friedrich-Loeffler-Str. 23c, D-17489 Greifswald, Germany
Abstract:Functional in vitro studies with isolated gastric mucosal cells require cytological identification of different cell types in suspension or primary culture. Since suitable techniques have not been well established, different staining methods for the discrimination of dispersed pig and guinea pig gastric cells have been developed on the basis of modified previous protocols for enzymatic cell dispersion. Chief and parietal cells were visualized by combined periodic acid-Schiff stains. Surface mucous and mucous neck cells were identified by affinity-labelling, using lectins with selective staining properties in situ. Two of the lectins were found to be specific markers for gastric polymorphonuclear cells. The following vital tests were found to be useful: succinic dehydrogenase for parietal cells, Nile blue/brilliant cresyl blue stains for chief cells, and different phagocytosis assays for endothelial cells and gastric phagocytes. Endocrine cells were characterized by immunocytochemistry using specific antibodies against gastrin, somatostatin, histamine and serotonin. The same technique using a vimentin antibody was performed for the identification of fibroblasts. Proliferation of mucosal cells in primary culture was monitored by the incorporation of bromo-deoxyuridine, which was subsequently detected by a monoclonal antibody.Some of these results were presented at the 7th International Conference on Experimental Ulcer, Berlin, Germany 1991 (see Giebel and Schwenk 1991)
Keywords:Stomach  Cell isolation  Cytology  Mucous cells  Chief cells  Parietal cells  Endocrine cells  Pig  Guinea pig
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