A novel tissue-slice culture model for non-malignant human prostate |
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Authors: | M Bläuer T L Tammela T Ylikomi |
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Institution: | (1) Department of Cell Biology, University of Tampere, Tampere, FI-33014, Finland;(2) Department of Urology, Tampere University Hospital, Tampere, FI-33014, Finland |
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Abstract: | A novel tissue culture system was established for modeling the non-neoplastic human prostate in vitro. Precision-cut prostate
slices were cultivated in culture plates with a gas-permeable base in a novel serum-free mixture. Cultivated specimens was
evaluated by an immunohistochemical analysis of cytokeratins 18 and 14, androgen receptor (AR), prostate specific antigen
(PSA), prostate acid phosphatase (PAP), and the endothelial cell marker von Willebrand factor. Epithelial viability in the
presence and absence of dihydrotestosterone (DHT) was also assessed. Satisfactory maintenance of glandular cytoarchitecture
was observed in the presence of DHT with approximately half of the glands displaying a columnar or cuboidal phenotype and
an intact layer of basal cells. In the absence of DHT, the corresponding percentage was significantly lower. The occurrence
of involutive changes and epithelial cell death was significantly higher in the absence of DHT. Glandular and stromal cells
maintained their capacity to express AR. PSA and PAP were expressed throughout the culture period, albeit at a lower level
than in uncultured tissue. The viability of endothelial cells differed markedly between individual samples. During culture,
the tissue slices became covered with epithelial cells originating from glands that were cut open during tissue slicing. This
cell layer consisted of a stratified basal compartment overlaid by cells with a luminal phenotype. The present culture system
provides a novel in vitro setting in which to study normal human prostate biology and pathobiology and may help to obviate
problems related to the use of established cancer cell lines and animal models.
This study was supported by grants from competitive research funding of the Pirkanmaa Hospital District, TEKES Drug 2000,
and the Juliana von Wendt Fund. |
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Keywords: | Prostate In vitro Tissue culture Serum-free medium Human |
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