Identical immunoreactivity of afferents to the rat suprachiasmatic nucleus with antisera against avian pancreatic polypeptide,molluscan cardioexcitatory peptide and neuropeptide Y |
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| Authors: | Dr Robert Y Moore Eric L Gustafson J Patrick Card |
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| Institution: | (1) Departments of Neurology, State University of New York at Stony Brook, Stony Brook, New York, USA;(2) Departments of Neurobiology and Behavior, State University of New York at Stony Brook, Stony Brook, New York, USA;(3) Departments of Psychology, State University of New York at Stony Brook, Stony Brook, New York, USA;(4) Department of Neurology, Health Sciences Center, SUNY & Stony Brook, T12, 020 Stony Brook, New York, USA |
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| Abstract: | Summary Avian pancreatic polypeptide (APP)-like, molluscan cardioexcitatory peptide (FMRF)-like and neuropeptide Y (NPY)-like immunoreactivities were studied in a secondary visual pathway in rat brain. The cell bodies of this pathway are located in the lateral geniculate nucleus and its terminal plexus is found in the suprachiasmatic hypothalamic nucleus (SCN). The neurons and terminal plexus demonstrated by antiserum to each peptide are identical, and immunoreactivity is blocked by preabsorption of each antiserum with a low concentration of the antigen against which it was raised. Immunoreactivity is also blocked by preabsorption of each antiserum with either NPY or APP. In contrast, APP- and NPY-like immunoreactivities are blocked only partially when these antisera are preabsorbed with concentrations of FMRF as high as 100  M. Since NPY is the only one of these peptides that has been isolated from mammalian brain, we conclude that NPY is the endogenous CNS peptide produced by neurons of the lateral geniculate-SCN projection. |
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| Keywords: | Avian pancreatic polypeptide Neuropeptide Y Molluscan cardioexcitatory polypeptide Suprachiasmatic nucleus Lateral geniculate nucleus Circadian rhythms |
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