首页 | 本学科首页   官方微博 | 高级检索  
   检索      


The effect of fixation conditions on the ultrastructural appearance of gastrin cell granules in the rat gastric pyloric antrum
Authors:N J McC Mortensen  Dr J F Morris
Institution:(1) Department of Anatomy, University of Bristol, The Medical School, Bristol, England;(2) Dept. of Anatomy, The Medical School, University Walk, BS8 1TD Bristol, England
Abstract:The ultrastructural appearance of gastrin cell (G cell) granules was studied after different fixation procedures. When the pH of prefixation was varied there was greater preservation of the electron density of granule cores after acidic (pH 5.0 and 6.0) than after neutral or alkaline (pH 7.0 and 8.0) prefixation. Increasing duration of prefixation at pH 7.3 resulted in progressive loss of electron density of the granule core with swelling and occasional rupture of the limiting membrane. In tissues where most granules had been rendered electron lucent by fixation, those granules remaining dense cored were preferentially located close to the Golgi zone. These findings indicate that the electron density of G cell granules is profoundly affected by conditions of fixation, and that immature granules are more resistant to loss of core density than mature granules. They also suggest that the gastrin granule in vivo, like other polypeptide granules, may have a "solid", osmotically inactive core.
Keywords:Gastrin cells  Electron microscopy  Fixation  Granule maturation
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号