Ultrastructural localization of immunolabeled substance P and methionine-enkephalin-octapeptide in the surface layer of the dorsal horn of rat spinal cord |
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Authors: | Shinsuke Katoh Setsuji Hisano Prof Dr Shigeo Daikoku |
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Institution: | (1) Department of Anatomy, School of Medicine, The University of Tokushima, Tokushima, Japan;(2) Department of Anatomy, School of Medicine, The University of Tokushima, 770 Tokushima, Japan |
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Abstract: | Summary A preembedding dual immunolabeling technique and electron microscopy were utilized to demonstrate the localization of immunoreactive substance P and methionine-enkephalin-octapeptide (Enk-8) in ultrathin sections of the surface layer (laminae I and II) of rat spinal dorsal horn. The immunoreaction of Enk-8 was visualized as goldtoned silver particles and that of substance P as diaminobenzidine reaction products. Axonal terminals with immunoreactive substance P, and also unlabeled axonal terminals, formed synaptic junctions with the perikarya and dendritic processes of Enk-8-containing neurons. Dendritic profiles immunolabeled for substance P were synaptically linked with unlabeled axons but not with Enk-8-positive ones. Furthermore, it was found that Enk-8 axons and substance P axons terminated synaptically in juxtaposition to one another on the same immunonegative dendrites. Among the Enk-8-containing neurons axonal profiles also appeared to be synaptically associated with immunoreactive Enk-8 dendritic processes. |
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Keywords: | Substance P Enkephalin Spinal dorsal horn Immunocytochemistry Electron microscopy Rat (Sprague-Dawley) |
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